Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method

A technology of skin regeneration and chitosan, applied in medical science, prosthesis, etc., can solve the problem of insufficient supply of autologous skin, achieve good elasticity and clinical ease of operation, promote healing, and prevent bacterial invasion

Inactive Publication Date: 2006-06-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method faces the disadvantage of insufficient supply of autologous skin. For

Method used

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  • Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method
  • Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method
  • Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0026] Collagen and chitosan were dissolved in 3% acetic acid solution respectively, and the preparation concentration was 0.5% collagen solution and 0.5% chitosan solution, and the chitosan solution was dropped into the collagen solution, wherein the chitosan The content is 10%, stir evenly and vacuum degassing; use the freeze-lyophilization method, freeze at -20°C for 2 hours, and then freeze-dry for 24 hours. Freeze-dried collagen / chitosan scaffolds were cross-linked in a vacuum oven at 105°C for 12 hours, cross-linked with 0.25% glutaraldehyde solution at 4°C for 24 hours, rinsed repeatedly with triple distilled water, and then frozen again - Lyophilization to obtain a cross-linked collagen-chitosan porous scaffold. On the surface of a silicone rubber film with a thickness of about 0.14 mm, a gelatin adhesive with good biocompatibility was added at 0.25 mg / cm 2 Evenly coated on the surface of the silicone rubber film, and then the collagen-chitosan porous scaffold was gen...

Embodiment 2

[0028] Collagen and chitosan were dissolved in 3% acetic acid solution respectively, and the preparation concentration was 0.5% collagen solution and 0.5% chitosan solution, and the chitosan solution was dropped into the collagen solution, wherein the chitosan The content is 10%, stir evenly and vacuum degassing; use the freeze-lyophilization method, freeze at -20°C for 2 hours, and then freeze-dry for 24 hours. Freeze-dried collagen-chitosan scaffolds were cross-linked in a vacuum oven at 105°C for 12 hours, cross-linked with 0.25% glutaraldehyde solution at 4°C for 24 hours, rinsed repeatedly with three-distilled water, and then frozen again - Lyophilization to obtain a cross-linked collagen-chitosan porous scaffold. On the surface of a silicone rubber film with a thickness of about 0.14 mm, α-cyanoacrylate octyl adhesive with good biocompatibility was added at 0.25 mg / cm 2 Evenly coated on the surface of the silicone rubber film, and then the collagen-chitosan porous scaff...

Embodiment 3

[0030]Collagen and chitosan were dissolved in 0.3% acetic acid solution respectively to prepare a 0.5% collagen solution and a 0.5% chitosan solution, and the chitosan solution was dropped into the collagen solution, wherein the chitosan The content is 5%, stir evenly and vacuum degassing; use freeze-lyophilization method, freeze to solid at -5°C, and then freeze-dry for 24 hours. Freeze-dried collagen / chitosan scaffolds were cross-linked in a vacuum oven at 80°C for 12 hours, cross-linked with 0.05% glutaraldehyde solution at 4°C for 1 hour, rinsed repeatedly with triple distilled water, and then frozen again - Lyophilization to obtain a cross-linked collagen-chitosan porous scaffold. On the surface of a silicone rubber film with a thickness of about 0.05 mm, α-cyanoacrylate octyl adhesive with good biocompatibility was added at 0.05 mg / cm 2 Evenly coated on the surface of the silicone rubber film, and then the collagen-chitosan porous scaffold was gently placed on the surfa...

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Abstract

A dual-layer scaffold for the skin regeneration is composed of a porous collagen-chitosan scaffold for inducing the migration, reproduction and differentiation of cells and the generation of dermal tissue and a silica rubber film as temporary epidermal layer for preventing the volatilization of water and the intrusion of bacteria. It has high biocompatibility, controllable degradation speed and excellent wound repairing power.

Description

technical field [0001] The invention relates to a double-layer skin regeneration support and a preparation method thereof. Specifically, it relates to a collagen-chitosan and silicon rubber double-layer skin regeneration support and a preparation method thereof. Background technique [0002] Burns or ulcers of the skin are very common surgical diseases, such as wounds in various accidents and wars, and skin ulcers caused by self-diseases such as diabetes. Skin defects can affect the patient's appearance in mild cases, and in severe cases can cause infection or a large loss of electrolytes and water, leading to the death of the patient. Although skin tissue has a strong regenerative ability, for a large area of ​​full-thickness skin defect, the skin loses its ability to spontaneously regenerate. The key to the treatment of full-thickness skin defects is to seal the wound as early as possible, so as to reduce the consumption of the body, the disturbance of the internal envir...

Claims

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Application Information

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IPC IPC(8): A61L27/60
Inventor 高长有马列石延超沈家骢韩春茂
Owner ZHEJIANG UNIV
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