Carbon dioxide pressurized sterilization method under normal temperature

A technology of carbon dioxide and sterilization methods, applied in chemistry and other fields, can solve the problems of inactivation of active substances in cells, decrease of intracellular pH, and no research reports, etc., and achieve the effect of easy volatilization, simple equipment, and reduced inactivation hydrolysis

Inactive Publication Date: 2006-06-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pressurized carbon dioxide will cause certain damage to the cell membrane, a small amount of intracellular substances leak out, and the cell membrane loses the ability to balance the pH value inside and outside the cell; after the accumulation of carbon dioxide in the cell exceeds the buffer ca

Method used

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  • Carbon dioxide pressurized sterilization method under normal temperature

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0017] Example 1

[0018] Escherichia coli was suspended in a solution containing 0.85% (mass percentage) NaCl and 0.1% (mass percentage) peptone to make a bacterial suspension, placed in an autoclave, adjusted the temperature in the autoclave to 35°C, and passed 7.8Mpa, 35 Keep pressure after carbon dioxide at ℃. After 15 minutes, the rate of viable bacteria (rate of viable bacteria = number of microorganisms after treatment / number of microorganisms before treatment) drops to 10 -1 , The viable bacteria rate after 30min is 10 -2.4 , The viable bacteria rate after 60min is 10 -4.2 .

[0019] 2. Sterilized by pressurized carbon dioxide with 180rpm stirring

Example Embodiment

[0020] Example 2

[0021] Suspend fresh Escherichia coli in a solution containing 0.85% (mass percentage) NaCl and 0.1% (mass percentage) peptone to make a bacterial suspension, place it in an autoclave, adjust the temperature in the autoclave to 35°C, and use a magnetic stirring speed of 180rpm, 7.8MPa, 35°C carbon dioxide was introduced and the pressure was maintained. The viable bacteria rate after 15min is 10 -1.8 , The viable bacteria rate after 30min is 10 -4.1 , The viable bacteria rate after 60min is 10 -7.8 . Comparing Example 1 and Example 2, it can be seen that stirring can improve the sterilization effect of pressurized carbon dioxide.

Example Embodiment

[0022] Example 3

[0023] Suspend fresh Escherichia coli in a solution containing 0.85% (mass percentage) NaCl and 0.1% (mass percentage) peptone to make a bacterial suspension, place it in an autoclave, adjust the temperature in the autoclave to 35°C, and use a magnetic stirring speed of At 180 rpm, 4.9 MPa, 35°C carbon dioxide was introduced and the pressure was maintained. The viable bacteria rate after 15min is 10 -0.4 , The viable bacteria rate after 30min is 10 -2.0 , The viable bacteria rate after 60min is 10 -5.1 , The viable bacteria rate after 90min is 10 -7.6 .

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PUM

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Abstract

The present invention discloses normal temperature pressurizing CO2 sterilization method. The sterilization method includes introducing CO2 to eliminate residual air from autoclave, filling liquid to be sterilized to at most 75 vol% of the autoclave, balancing the temperature of the autoclave in water bath at 25-45 deg.c for 10-15 min, introducing CO2 at 2-8 MPa and 25-45 deg.c, and maintaining the pressure for 5-120 min. The present invention has broad spectrum sterilization of killing Gram+ bacterium, Gram- bacterium, saccharomycetes, mildew and other microbes. The normal temperature operation is suitable for sterilizing heat sensitive matter and maintaining food flavor, and lowering pressure while prolonging the pressure maintaining period can reduce the deactivation and hydrolysis of enzyme, protein and other matters. Small amount of alcohol may be added to raise the sterilization effect, save power and reduce operation period.

Description

technical field [0001] The invention relates to a normal temperature and pressurized carbon dioxide sterilization method. Background technique [0002] In the food industry and biotechnology, it is often necessary to sterilize the target system. The most commonly used sterilization method is heat sterilization. The biggest disadvantage of this method is that it is easy to inactivate heat-labile substances such as proteins, amino acids, and enzymes, and also affects the flavor of some foods. Therefore, it is necessary to develop a normal temperature sterilization method. The room temperature sterilization methods that are currently available or are being developed include ultraviolet sterilization, chemical sterilization, ultra-high pressure sterilization, radiation or high-energy electronic sterilization, and oscillating magnetic field sterilization. In 1987, Kamihira et al. found that carbon dioxide under a certain pressure has the effect of killing some microorganisms. Us...

Claims

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Application Information

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IPC IPC(8): A61L2/20A61L2/18
Inventor 姚善泾吴垠关怡新林东强
Owner ZHEJIANG UNIV
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