Process for producing chloromycetin molecular engram solid phase extraction small column

A solid-phase extraction cartridge and molecular imprinting technology, applied in the field of bioengineering, can solve the problems of irregular particle shape, uneven particle size of molecularly imprinted polymer, poor enrichment effect of trace chloramphenicol, etc., and achieve high efficiency. Effect

Inactive Publication Date: 2006-08-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that there are disadvantages such as uneven particle size and irregular particle shape of molecularly imprinted polymers, and the enrichment effect on trace amounts of chloramphenicol is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of chloramphenicol molecularly imprinted polymer microspheres

[0023] (1), the molar ratio of template molecule, functional monomer and crosslinking agent is 1:2; the volume ratio of the total volume of diluent and crosslinking agent to water is 1:6; the concentration of polyvinyl alcohol 1788 (PVA1788) 3%; 0.8ml functional monomer N,N-diethylaminoethyl methacrylate (DAM) and 1mmol template molecule chloramphenicol were dissolved in the diluent, and ultrasonicated for 5min; Alcohol dimethacrylate (EGDMA), 120mg initiator (AIBN) and 15ml diluent (octanol 10ml, chloroform 5ml) were mixed, ultrasonically acted on for 5-10min; the above three solutions were mixed, at a constant temperature of 60°C and a constant speed of 350rpm Stir for 20 hours; (2) Stir the molecularly imprinted polymer microspheres prepared by the above reaction in 80°C water for 60 minutes, then cool down and filter, wash with double distilled water for 3 times after filtering, then was...

Embodiment 2

[0036] (1) Preparation of chloramphenicol molecularly imprinted polymer microspheres

[0037](1), the molar ratio of template molecule, functional monomer and cross-linking agent is 1: 4; The total volume of diluent and cross-linking agent and the volume ratio of water are 1: 8; Polyvinyl alcohol 1788 (PVA1788) The concentration is 4%; 0.8ml functional monomer N,N-diethylaminoethyl methacrylate (DAM) and 1mmol template molecule chloramphenicol are dissolved in the diluent, and ultrasonically acted for 5-10min; Agent ethylene glycol dimethacrylate (EGDMA), 120mg initiator (AIBN) and 15ml diluent (octyl alcohol 10ml, chloroform 5ml) were mixed, and ultrasonication was applied for 5-10min; the above three solutions were mixed and kept at a constant temperature of 65°C Stir at a constant speed of 400rpm for 22h; (2) Stir the molecularly imprinted polymer microspheres prepared by the above reaction in 80°C water for 60min, then cool down and filter, wash with double distilled water...

Embodiment 3

[0044] (1) Preparation of chloramphenicol molecularly imprinted polymer microspheres

[0045] (1), the molar ratio of template molecule, functional monomer and crosslinking agent is 8: 25; The total volume of diluent and crosslinking agent and the volume ratio of water are 114; The concentration of polyvinyl alcohol 1788 (PVA1788) is 6%; 0.8ml functional monomer N,N-diethylaminoethyl methacrylate (DAM) and 1mmol template molecule chloramphenicol were dissolved in the diluent, and ultrasonicated for 10min; 5ml crosslinking agent ethylene glycol Dimethacrylate (EGDMA), 120mg initiator (AIBN) and 15ml diluent (octanol 10ml, chloroform 5ml) were mixed, and ultrasonically applied for 10min; the above three solutions were mixed, and stirred at a constant temperature of 75°C and 450rpm for 24h; (2) Stir the molecularly imprinted polymer microspheres prepared by the above reaction in 80°C water for 60 minutes, then cool down and filter, wash with double distilled water 3 times after f...

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Abstract

The present invention relates to a preparation method of chloromycetin molecular engram solid-phase extraction small column, belonging to the field of biological engineering technology. Said method includes the following steps: using template molecule chloromycetin, functional monomer N-N-diethylaminoethyl methacrylate and cross-linking agent ethylene glycol dimethacrylate according to the mole ratio of 1:2-8:25 and making them undergo the process of suspension polymerization to obtain chloromycetin molecular engram polymer microsphere; weighing said chloromycetin molecular engram polymer microsphere and filling it into polyprophylene solid-pase extraction small column so as to obtain the invented chloromycetin molecular engram solid-phase extraction small column.

Description

technical field [0001] The present invention relates to a method in the technical field of bioengineering, in particular to a molecularly imprinted chloramphenicol solid for highly selective separation, purification and enrichment of chloramphenicol residues in animal-derived foods and aquatic products. Preparation method of phase extraction cartridge. Background technique [0002] Chloramphenicol (CAP) is a broad-spectrum antibiotic isolated from the culture medium of Streptomyces Venezuela for the first time in 1947, and has inhibitory effects on both Gram-positive and Gram-negative bacteria. However, due to the nitro group on its benzene ring, its decomposition half-life is long and has serious side effects. There are many detection methods for chloramphenicol residues. At present, developed countries have more and more stringent requirements for its detection limit. , the European Union dropped from the original 10μg / kg to 0.1μg / kg, and the detection limit stipulated by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/56G01N30/60B01J20/281
Inventor 张大兵武爱波史西志李荣秀
Owner SHANGHAI JIAO TONG UNIV
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