Method for detecting Benzhou's protein in urine

Abstract

This invention discloses a method for testing if there are Gones-proteins in urine, which applies a centrifugal method realized under the lab condition to replace the filter method needing heat preservation to deposit and separate the Gones-proteins, which greatly reduces the influence of temperature to the test result. Since this invention eliminates albumins and globins and heats Gones-proteins only, it increases its dissolution ability and is in favor of getting more accurate test result.

Description

technical field [0001] The invention relates to a biochemical analysis method, in particular to a method for detecting whether there is Ben's protein in urine. Background technique [0002] Zhou's protein, also known as condensed protein, is a light chain or copolymer of immunoglobulin. This protein precipitates when heated to 40-60°C under a certain pH value, and the temperature rises to 100°C. The precipitation disappears at ℃, and the precipitation reappears when it is cooled again. According to this principle, it can be detected whether there is Zhou's protein in the urine. [0003] The detection method in the prior art is: take 4ml of urine in a test tube, add 1ml of acetate buffer, mix well, place it in a water bath at 56°C for 15 minutes, if there is turbidity or precipitation, put the test tube into Boil in boiling water for 3 minutes, and observe the changes in turbidity or precipitation in the test tube. If the turbidity becomes clear, weakens, or the precipitatio...

AI Technical Summary

Problems solved by technology

[0004] The disadvantage of this method is that when the total protein content in the urine is above 5g / L, because the protein content in the urine is too high, some Zhou’s proteins cannot be completely dissolved when heated above 90°C, resulting in coagulation. The change of dissolution phenomenon is not obvious, and the result is difficult to judge

In addition, multiple myeloma is generally seen in kidney damage, causing a large amount of globulin and albumin to enter the urine. In order to overcome their interference, it needs to be filtered out. It is necessary to maintain a constant temperature during filtration, because if the temperature drops, it will cause Zhou’s protein to precipitate. It is filtered out, but it is difficult to maintain a constant temperature when filtering under laboratory conditions, so it will also affect the accuracy of the test results