The invention discloses a chicken whole blood simple, convenient and quick DNA extraction method which comprises the following steps of after adding chicken whole blood in an anticoagulant, adding a mixture in lysate for mixing, putting a mixture in a water solution boiler with the temperature being 56-60 DEG C for 20-30min, adding a separating medium in a product, putting a mixture in the water solution boiler with the temperature being 70-80 DEG C for 20-30min for centrifugation, taking a supernate, transferring the supernate into another EP pipe, adding a precipitated solution, performing centrifugation, reserving precipitates, adding a washing solution, and performing uniform mixing and centrifugation; and carefully discarding the supernate, collecting the precipitates, drying the precipitates at the room temperature, and adding a TB buffer liquid to obtain a chicken DNA extracting solution. By adopting the chicken whole blood simple, convenient and quick DNA extraction method provided by the invention, the purity and the integrity of extracted DNA can both reach test requirements, and the concentration and the purity are superior to that of DNA extracted by an existing kit. Bycombining with application on a chicken molecular breeding aspect, proved that the method is an environment-friendly DNA extraction method with low cost and simple operation.