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Phosphorus starvation induced gene promoter and its application

A technology of phosphorus deficiency induction and promoter, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of growth and development damage, low utilization efficiency of phosphorus fertilizer, and difficulty in grasping the time of fertilization.

Inactive Publication Date: 2006-10-11
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to grasp the accurate fertilization time in field production, and the method of applying base fertilizer is usually used
Due to the leaching of rainwater and the fixation of soil, the utilization efficiency of phosphorus fertilizer is very low, which often cannot meet the needs of plants in the later stage of development
Fertilize when the plants show obvious phosphorus deficiency symptoms, and the time is too late, and some growth and development damages that have been caused cannot be remedied

Method used

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  • Phosphorus starvation induced gene promoter and its application
  • Phosphorus starvation induced gene promoter and its application
  • Phosphorus starvation induced gene promoter and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Acquisition of Phosphorus Deficiency Inducible Promoter and Its Functional Verification

[0021] 1. Phosphorus deficiency inducible promoter P PDI1 the acquisition

[0022] According to the genome sequence of Arabidopsis thaliana, the specific primer F: 5′- AAG CTT GTTTCA GTA CTA GCC TCA CG-3' and R:5'- GGA TCC CAA AAT ATA AAC TCA AG-3', a HindIII and BamHI restriction site were added to the 5' end of the primer respectively. The genomic DNA of the columbia ecotype Arabidopsis thaliana was extracted as a template, and the promoter fragment was amplified by PCR; wherein, the amplification system was DNA 2 μl (10ng), 10x ExTaq buffer 2.5 μl, dNTP (2.5mM) 2.0 μl, primer F (10μM) 0.5μl, primer R (10μM) 0.5μl, ExTaq (5u / μl) 0.2μl, H 2 O 17.3 μl, the amplification program was: 94°C pre-denaturation for 5 minutes; 35 cycles of 94°C for 1 minute, 55°C for 1 minute, 72°C for 2 minutes; 72°C for 5 minutes. A 1749bp fragment was amplified and connected to the p...

Embodiment 2

[0033] Embodiment 2, use phosphorus deficiency to induce promoter P PDI1 Phosphorus nutrition status of early warning plants

[0034] due to P PDI1 The expression is exclusively induced by phosphorus starvation and can be regulated by phosphorus signals, so the promoter can be fused with some target genes to construct economically valuable vectors.

[0035] Because of P PDI1 The induction strength of the promoter was enhanced with the decrease of phosphorus concentration. When the phosphorus concentration in the medium was 250 μM, P PDI1 A promoter initiates the expression of a gene. According to the abundance and deficiency index of soil available phosphorus, 10mg / Kg is not deficient. That is, when the soil available phosphorus is moderate or lacking, the PDI1 gene promoter P PDI1 Expression of the controlled gene will be initiated. If P PDI1 The promoter is fused with a pigment gene and transferred into the plant. When the transgenic plant is subjected to moderate pho...

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Abstract

The invention discloses a kind of lacking phosphorus induction gene promoter and application. The promoter has one of the nucleotide sequences as follow: 1) DNA sequences of SEQ ID NO.1 in sequence table; 2) the nucleotide sequence that can hybrid with restricted DNA sequences of SEQ ID NO.1 in sequence table at high strict condition. The invention's lacking phosphorus induction promoter express only when lack phosphorus, control by phosphorus signal, therefore, the promoter can be used to fuse with target gene to construct the economic value vector.

Description

technical field [0001] The invention relates to a phosphorous deficiency inducible gene promoter and application thereof. Background technique [0002] Phosphorus is one of the macronutrients necessary for plant growth. It is not only an important component of ATP, nucleotides and phospholipids in plant cells, but also plays a very important role in regulating energy transfer, protein activation and carbon and nitrogen metabolism. . The absolute phosphorus content in the soil is usually high (>1000μM), but phosphorus is easily fixed in organic and inorganic forms in the soil, so the concentration of available phosphorus is very low, about 2-10μM, and the phosphorus diffused to the root surface is even lower. It is difficult to meet the needs of plant growth and development. In agricultural production, a large amount of phosphorus fertilizer is used to meet the needs of crop growth. However, it is difficult to grasp the accurate fertilization time in field production, a...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82
Inventor 赵婷凌宏清
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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