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Ginkgo leaf extract and production process of separating high purity effective component of the extract

A technology of ginkgo biloba and ginkgolide, applied in the field of biomedicine, can solve the problems of cumbersome operation, large loss of active ingredient types and quantities, etc., and achieve the effect of simple process, high efficiency and avoiding waste

Inactive Publication Date: 2006-10-18
厦门国宇知识产权研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To separate the two components, more steps are required, the operation is cumbersome, and the type and quantity of active ingredients are lost.

Method used

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  • Ginkgo leaf extract and production process of separating high purity effective component of the extract
  • Ginkgo leaf extract and production process of separating high purity effective component of the extract
  • Ginkgo leaf extract and production process of separating high purity effective component of the extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add 120L of water to 12kg of dry ginkgo leaf powder and boil for 0.5h. Filter, add 60L and 36L water to the filter residue successively and boil for 0.5h respectively, and combine the three extracts. 12kg of pre-treated AB-8 macroporous adsorption resin (product of Tianjin Nankai University Chemical Plant) was placed on the water extraction solution, and the column flow rate of the water extraction solution was controlled to be 1 times the resin column volume / h. After all the extracts are applied to the column, the resin column is eluted with distilled water of 8 times the volume of the resin column, and the flow rate of the eluent is controlled to be 3 times the volume of the resin column / h until the effluent is colorless, and then eluted with 20% ethanol to The effluent was colorless.

[0037] The resin column continued to be eluted with 1% NaOH solution until the effluent was colorless, and the dilute alkali eluate was collected, and the pH was adjusted to 3 with 6N...

Embodiment 2

[0047] Add 100L of water to 10kg of dry ginkgo leaf powder and boil for 1h. Filter, add 50L and 30L water to the filter residue successively and boil for 1 hour respectively, and combine the three extracts. 10kg of pre-treated D-101 macroporous adsorbent resin (product of Tianjin Nankai University Chemical Plant) was placed on the water-extracted liquid, and the flow rate of the water-extracted liquid was controlled to be 2 times of resin column volume / h. After all the extracts are applied to the column, the resin column is eluted with distilled water of 5 times the volume of the resin column, and the flow rate of the eluent is controlled to be 1 times the volume of the resin column / h, until the effluent is colorless, and then washed with 25% ethanol. Take off until the effluent is colorless.

[0048] The resin column continued to be eluted with 0.5% NaOH solution until the effluent was colorless, and the dilute alkali eluate was collected, and the pH was adjusted to 4 with 6...

Embodiment 3

[0058] To 1kg commercially available Ginkgo biloba crude extract (Zhejiang Kangenbei Pharmaceutical Co., Ltd. product, trade name is " Ginkgo biloba crude extract ", this Ginkgo biloba crude extract contains about 24% of total flavonoids of Ginkgo biloba, and about 24% of total terpene lactones of Ginkgo biloba 6%), add 100L of water, stir to dissolve, and filter. 50kg of pre-treated S-8 macroporous adsorption resin (product of Tianjin Nankai University Chemical Factory) was placed on the water extraction solution, and the column flow rate of the water extraction solution was controlled to be 1 times the volume of the resin column / h. After all the extracts are applied to the column, the resin column is eluted with distilled water of 5 times the volume of the resin column, and the flow rate of the eluent is controlled to be 0.5 times the volume of the resin column / h until the effluent is colorless, and then eluted with 18% ethanol to The effluent was colorless.

[0059] The re...

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PUM

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Abstract

The present invention discloses new process of extracting and separating high purity bilobalide and ginkgetin. By means of macroporous adsorption resin chromatographic separation technology, the water extract of ginkgo material is eluted in macroporous adsorption resin column successively with distilled water, 5-25 % concentration alcohol solution, 0.01-10 % concentration alkali solution, distilled water and 30-95 % concentration alcohol solution, and the elute of alkali solution and the elute of 30-95 % concentration alcohol solution are collected separately, concentrated and purified to obtain bilobalide or ginkgetin. The process has high efficiency, simple operate, low production cost and environment friendship, and the product has high quality and high yield.

Description

technical field [0001] The present invention relates to a method for extracting and separating active ingredients of medicine from plants, in particular to a method for extracting and separating high-purity ginkgolides and ginkgo flavonoids from ginkgo leaves or ginkgo leaf crude extracts by using macroporous adsorption resin chromatographic separation technology The method belongs to the field of biomedicine. Background technique [0002] Ginkgo biloba L., also known as Gongsun tree, is one of the oldest Mesozoic relict plants. It is known as the "living fossil" of gymnosperms and is a special product in my country. my country's ginkgo resources account for about 70% of the world's total. The main active ingredients in Ginkgo biloba are flavonoids and terpene lactones, which can expand coronary blood vessels, improve cerebral circulation, eliminate oxygen free radicals, and inhibit platelet-activating factor (PAF). For the treatment of coronary heart disease, cerebral thr...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D493/22
Inventor 郭东宇
Owner 厦门国宇知识产权研究有限公司
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