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Method for detecting inorganic phosphorus in milk

A technology for inorganic phosphorus and milk, applied in the field of detection of breast milk, inorganic phosphorus in milk, and inorganic phosphorus in milk or goat milk, to achieve great social value, increase nutritional value, and small error

Inactive Publication Date: 2006-11-22
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people at home and abroad have only paid attention to the determination of inorganic phosphorus in urine and serum, and rarely mentioned the method of determining inorganic phosphorus in milk.

Method used

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  • Method for detecting inorganic phosphorus in milk
  • Method for detecting inorganic phosphorus in milk
  • Method for detecting inorganic phosphorus in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take breast milk from a mother who has given birth for 3 months and is breastfeeding, and centrifuge it on a TGL-16B high-speed desktop centrifuge to obtain the supernatant (a large amount of fat is separated); take 6ml of the above supernatant, transfer it to a 10ml ultrafiltration cup, and store it at room temperature and 5×10 5 Under the nitrogen condition of Pa, use the PM-10 ultrafiltration membrane with a molecular weight cut-off of 10kD, ultrafilter with Amicon Model 8010 ultrafilter, collect the colorless clear liquid that filters out (collect 3 / 4 of the former milk volume), in- Store at 17°C to -20°C for measurement; prepare HEPES (10mM) buffer solution with pH 7.0, and prepare 2×10 -3 MYbCl 3 solution and 2 x 10 -3 Catechol violet solution of M; add 2ml of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 50 μl of catechol violet solution of M was added to the cuvette, at this time the solution turned from colorless to yellow, detected on...

Embodiment 2

[0018] Embodiment 2: prepare the HEPES (10mM) buffer solution of pH6.5, all the other are with embodiment 1, obtain the inorganic phosphorus content in the mother's milk and be 2.42mmol / L, the ultraviolet-visible absorption figure sees figure 2 .

Embodiment 3

[0019] Embodiment 3: with Yb (ClO 4 ) 3 The solution, get another people's breast milk, all the other are with embodiment 1, The content of inorganic phosphorus in breast milk is 2.34mmol / L, and the ultraviolet-visible absorption diagram is shown in image 3 .

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Abstract

The detection method for inorganic P content in milk by UV visible spectrophotometer in HEPES buffer with 6.5-7.5 pH value and Yb ion and pyrocatechol violet as developer is benefit to learn supply condition and improve production quality. This invention is simple and fit to clinic an industry.

Description

Technical field: [0001] The invention relates to a method for detecting inorganic phosphorus, in particular to a method for detecting inorganic phosphorus in milk, that is, a method for detecting inorganic phosphorus in breast milk, milk or goat milk. Background technique: [0002] For newborn babies, breast milk or cow's milk or goat's milk is the most important food source for their growth and development. And precisely this period is the fastest growing period for children. They are in the period of vigorous bone formation and the stage of immature brain and intelligence. They need to take in appropriate nutrients to ensure normal growth and development, and to prevent malnutrition, rickets, anemia etc. Ionized elements such as calcium and phosphorus are necessary for normal blood coagulation and neuromuscular function, and because they are important components of bone, they are also called macroelements. However, if the ratio of absorbed calcium and phosphorus is out o...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28G01N21/78
Inventor 阴彩霞霍方俊杨频
Owner SHANXI UNIV
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