The invention provides an electric power system stability control strategy on-line simulation calibration method. The method comprises the steps that step 1, electric power system operation data are loaded; step 2, traversal is conducted on items of a stability control strategy index table, and the stability control strategy index table respectively has indexing relationship with a mode constraining table, a monitoring constraining table, a fault constraining table, a posterior constraining table and a stability control describing table; when the items of the stability control strategy index table are traversed, whether operation data meet the mode constraining, the monitoring constraining, the fault constraining and the posterior constraining of one item is sequentially judged, if yes, step 3 is executed; if not, step 4 is conducted; step 3, transient stability simulation is conducted on a stability control action corresponding to the item, if the system is stable, the stability control action is judged to be the stability control strategy suitable for an electric power system, otherwise the step 4 is executed; step 4, pre-warning information is given. The electric power system stability control strategy on-line simulation calibration method used as a comprehensive assessment method is standard and precise in strategy description, and is rapid and efficient in matching analysis.