Preparation method of herbicide imazethapyr high efficiency degradation bacterial agent

A high-efficiency technology for degrading bacteria and herbicide imidazole, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems such as low degradation rate, impact, and impact on development

Inactive Publication Date: 2006-11-29
NORTHEAST AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Although the microbial degradation of pesticides has been greatly developed at present, and various microbial strains (including bacteria, fungi, etc.) Affected by lower degradation rate
Biodegradati

Method used

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Examples

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Embodiment Construction

[0022] The following examples describe the present invention in more detail:

[0023] 1. Take the soil that has been applied with imazethapyr for many years in the field as the treatment object, and dissolve it with sterile saline to obtain a soil suspension;

[0024] Add imazethapyr to the peptone medium at a concentration of 100mg L -1 After sterilizing and cooling the culture medium at 121°C for 20 minutes, inoculate the soil suspension with an inoculum size of 10%, shake the flask at 30°C for 5 days, and inoculate the culture solution with imazethapyr at a concentration of 10% inoculum. 200mg·L -1 peptone medium, and then cultured in shake flasks at 30°C for 5 days, and inoculated 10 times by analogy, with imazethapyr 100mg·L -1 The concentration gradient increases, so that the concentration of imazethapyr in the final peptone medium reaches 1000mg·L before inoculation -1 , to obtain enriched and degraded strain culture seeds;

[0025] The seeds of the enriched degradi...

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PUM

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Abstract

The invention provides a producing method for high-efficiency degradation bacterial agent of herbicide imidazole acetic niacin bacterium. The soil that uses imidazole acetic niacin for several years is used as the dealing object to get the soil suspending liquid; putting the 100mgL-1 thickness imidazole acetic niacin into the peptone culture medium, after cooling, inoculating soil suspending liquid, and the inoculum size is 10%, whisking and cultivating for 5 days; inoculating the cultivating liquid into 200mgL-1 thickness imidazole acetic niacin peptone culture medium, whisking and cultivating for 5 days; repeating the process for ten times, and each time improving the thickness of imidazole acetic niacin 100mgL-1 to gain the thickness of 1000mgL-1 imidazole acetic niacin in peptone culture medium. The enrichment degradation strain cultivating seed is gained that would be made into product after taking slant cultivation, first stage expanding cultivation, second stage expanding cultivation, and adding degradation strain culture medium, fermenting, and packaging. The product can effectively handle with the damage to sensitive plant caused by imidazole acetic niacin.

Description

(1) Technical field [0001] The invention relates to a preparation method of a biodegradation agent, in particular to a preparation method of a biodegradation agent capable of degrading the herbicide imazethapyr. (2) Background technology [0002] Imazethapyr belongs to imidazolinone herbicides and is a high-efficiency, low-toxicity, broad-spectrum soybean field herbicide developed by BASF in the 1980s. Since it was used on a large scale in the 1990s, it has been the main variety of chemical weeding in soybean fields due to its broad herbicidal spectrum, ultra-high activity, high selectivity, and flexible use methods. According to preliminary statistics, in 2005, the soybean planting area in Heilongjiang Province was 50.5 million mu, and the sales volume (preparation) of imazethapyr reached more than 4,000 tons. Imidazolinone herbicides are non-volatile and non-hydrolyzed, and disappear mainly through microbial degradation in the soil. The residual period is relatively long,...

Claims

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Application Information

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IPC IPC(8): C12N1/00
Inventor 陶波滕春红栾凤侠刘辉金萍
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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