Preparation method of herbicide imazethapyr high efficiency degradation bacterial agent

Abstract

The invention provides a producing method for high-efficiency degradation bacterial agent of herbicide imidazole acetic niacin bacterium. The soil that uses imidazole acetic niacin for several years is used as the dealing object to get the soil suspending liquid; putting the 100mgL-1 thickness imidazole acetic niacin into the peptone culture medium, after cooling, inoculating soil suspending liquid, and the inoculum size is 10%, whisking and cultivating for 5 days; inoculating the cultivating liquid into 200mgL-1 thickness imidazole acetic niacin peptone culture medium, whisking and cultivating for 5 days; repeating the process for ten times, and each time improving the thickness of imidazole acetic niacin 100mgL-1 to gain the thickness of 1000mgL-1 imidazole acetic niacin in peptone culture medium. The enrichment degradation strain cultivating seed is gained that would be made into product after taking slant cultivation, first stage expanding cultivation, second stage expanding cultivation, and adding degradation strain culture medium, fermenting, and packaging. The product can effectively handle with the damage to sensitive plant caused by imidazole acetic niacin.

Description

(1) Technical field [0001] The invention relates to a preparation method of a biodegradation agent, in particular to a preparation method of a biodegradation agent capable of degrading the herbicide imazethapyr. (2) Background technology [0002] Imazethapyr belongs to imidazolinone herbicides and is a high-efficiency, low-toxicity, broad-spectrum soybean field herbicide developed by BASF in the 1980s. Since it was used on a large scale in the 1990s, it has been the main variety of chemical weeding in soybean fields due to its broad herbicidal spectrum, ultra-high activity, high selectivity, and flexible use methods. According to preliminary statistics, in 2005, the soybean planting area in Heilongjiang Province was 50.5 million mu, and the sales volume (preparation) of imazethapyr reached more than 4,000 tons. Imidazolinone herbicides are non-volatile and non-hydrolyzed, and disappear mainly through microbial degradation in the soil. The residual period is relatively long,...

AI Technical Summary

Problems solved by technology

Although the microbial degradation of pesticides has been greatly developed at present, and various microbial strains (including bacteria, fungi, etc.) Affected by lower degradation rate

Biodegradation is the development direction to solve pesticide residues. Although this method is green and environmentally friendly, it affects the development of this technology due to its low degradation rate.