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Process for preparing blumea balsamifera total flavone extract and blumea balsamifera flavone extract

A technology for extracting and total flavonoids, which is applied in the field of improved preparation of total flavonoids of Ainaxiang, can solve the problems of complicated experimental research on processing steps and difficulty in applying large-scale industrial production, etc.

Active Publication Date: 2007-01-24
SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this field, the dry loading method can not be affected by the loading solvent, but due to the cumbersome processing steps, the dry loading method is generally limited to experimental research, and it is difficult to apply to large-scale industrial production

Method used

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  • Process for preparing blumea balsamifera total flavone extract and blumea balsamifera flavone extract
  • Process for preparing blumea balsamifera total flavone extract and blumea balsamifera flavone extract
  • Process for preparing blumea balsamifera total flavone extract and blumea balsamifera flavone extract

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0055] Thereby, the present invention provides a kind of preparation method of the total flavonoid extract of Ai Naxiang improved, in this method, Ai Naxiang extract enriches flavonoid composition by polyamide adsorption chromatography; , the sum of the total mass of flavonoids accounts for 50% to 95% of the total mass of the extract; preferably, the sum of the total mass of flavonoids accounts for 60% to 95% of the total mass of the extract; more preferably, its The sum of the total mass of flavonoids accounts for 75%-95% of the total mass of the extract.

[0056] The preparation method of the total flavonoids of Ainaxiang of the present invention may comprise the following steps:

[0057] (1) Ainaxiang medicinal material is extracted with 20%-80% (v / v) ethanol aqueous solution;

[0058] (2) After pretreatment, the Ainaxiang extract in step (1) is centrifuged or filtered to obtain a clarified extract;

[0059] (3) The extract from step (2) is loaded on a polyamide chromatog...

Embodiment 1

[0084] [Example 1] Ainaxiang extract

[0085] Ainaxiang medicinal material: the medicinal material in the planting base of Luodian County, Guizhou Province (batch number 021015), which has been identified as the dried stem and leaf of Ainaxiang Blumea balsamifera (L.) DC of Compositae.

[0086] Determination of the total flavonoids content of medicinal materials: 100g of Ainaxiang medicinal materials were chopped, soaked in 1L of 75% ethanol for 24 hours, installed percolation column (φ8cm, l100cm), percolation with 75% ethanol, percolation flow rate 20ml / min, interval sampling for HPLC -DAD detection until the percolation liquid is substantially free of flavonoids.

[0087] Extraction: 10 parts of Ainaxiang medicinal material 100g, chopped, extracted with 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, and 10% ethanol as the extraction solvent, That is, each portion of the medicinal materials was reflux extracted with 1.2 L of ethanol of different concentrations mentioned above...

Embodiment 2

[0093] [Example 2] The polyamide column bed adsorption of Ainaxiang extract

[0094] Material:

[0095] (1) Polyamide: produced by Changzhou Changfeng Chemical Co., Ltd., used for column chromatography, particle size 60-100 mesh. Pretreatment according to the product instructions.

[0096] (2) Others: with embodiment 1.

[0097] extract:

[0098] (1) 10 parts of Ainaxiang medicinal material 100g, chopped, extracted with 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% ethanol as extraction solvent , that is, each part of the medicinal materials was extracted 3 times with 1.2 L of ethanol with different concentrations above, and each time was 2 hours. The extracts were combined to obtain the extract (I).

[0099] (2) 4 parts of 100g Ainaxiang medicinal material, chopped, and extracted with 60%, 50%, 40%, 30% ethanol as extraction solvent respectively, that is, each part of the medicinal material was extracted 3 times with 1.2L of ethanol with different concentrations above...

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Abstract

The present invention relates to improved process of preparing Blumea balsamifera total flavone extract and is especially the process of preparing effective total flavone component from Blumea balsamifera plant material. The improved process includes proper pre-treatment step and subsequent chromatographic separation with polyamide as adsorbent. The improved process has obviously raised total flavone preparing efficiency, lowered preparation cost, and results in the reduced dosage.

Description

technical field [0001] The present invention relates to an improved method for preparing the total flavonoids extract of Aiinaxiang. The method is an improvement to the preparation method disclosed in Chinese patent application CN1403451, and the entire text of the patent application is incorporated in this specification as a reference. Background technique [0002] Ainaxiang is the dry stem and leaf of Blumea balsamifera (L.) DC. Ainaxiang, also known as Dafengai in traditional Chinese medicine. "Guangxi Standards of Chinese Medicinal Materials" (1990 edition) records that Ainaxiang is "pungent, bitter, and warm" in nature, and has the functions of "warming the middle and activating blood circulation, regulating menstruation, and expelling wind and dampness". For the treatment of "exogenous cold, diarrhea, abdominal pain, borborygmus, swelling, irregular menstruation, pain in muscles and bones" and other diseases. [0003] The leaves of Aina japonica contain volatile oils...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P9/10A61P3/06C07D311/30C07D311/32C07H1/08C07B63/00A61K127/00A61K135/00
Inventor 赵金华康晖曾伟珍姚光辉殷米来张丽娟李靖冯汉林于琳
Owner SHENZHEN NEPTUNUS PHARMA RES INST CO LTD