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Chemical light emitting method and its device for high snesitivity detecting micro albumin

A microalbumin and chemiluminescence technology, applied in the field of microalbumin detection, can solve the problems of expensive instruments, complicated operations, and high detection costs, and achieve the effects of low cost, high sensitivity, and improved luminescence intensity.

Inactive Publication Date: 2007-01-24
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have relatively high sensitivity, the operation is relatively complicated, the instruments used are expensive, and the detection cost is high.

Method used

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  • Chemical light emitting method and its device for high snesitivity detecting micro albumin
  • Chemical light emitting method and its device for high snesitivity detecting micro albumin
  • Chemical light emitting method and its device for high snesitivity detecting micro albumin

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Embodiment

[0034] figure 1 The specific structure of the device of the present invention is shown, by figure 1 It can be seen that the high-sensitivity detection microalbumin chemiluminescence device includes: a dark room 1, a transparent ultra-thin sample cell 2, a microsampler 3, an integrating sphere 4, a chemiluminescence receiving component, an electrical pulse processing component, a computer 10, a power supply 11, The voltage regulator 12 of the photomultiplier tube; the transparent ultra-thin sample cell 2, the microsampler 3, the integrating sphere 4, and the chemiluminescence receiving component are arranged in the dark room, and the chemiluminescence receiving component and the electric pulse processing component are sequentially connected with the computer 10 Connection; the micro sampler 3 is suspended on the sample cell 2; the sample cell 2 is fixed on the spherical wall of the integrating sphere 4; the chemiluminescence receiving component, the computer 10 and the power su...

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Abstract

The method includes steps: chemical reaction produces stable single oxygen source; reaction between single state oxygen and high selectivity, high sensitive chemiluminescence probe produces intermediate product in high energy, and sending out photons caused by deexcitation quickly; albumin in minute quantities combined with FCLA enhances sensitivity of chemiluminescence intensity; it is linear relation between magnitude of chemiluminescence enhanced sensitivity and concentration of albumin; using change of luminous intensity corresponding to change of concentration of albumin can detect albumin in trace amount. Equipment for implementing the method includes darkroom, sample cell, sample injector in minute quantities, integrating sphere, module for receiving chemiluminescence, module for treating electrical pulse, and computer etc. Features are: small sample needed, short time consumption, low cost, high sensitivity, and certain degree of interference immunity.

Description

technical field [0001] The invention relates to a technology for detecting microalbumin, in particular to a chemiluminescence method and a device for detecting microalbumin with high sensitivity. Background technique [0002] Serum albumin (HSA) is the most abundant protein in serum, accounting for about 40% to 60% of the total serum protein. It plays an important role in the transport of drugs and the maintenance of normal blood osmotic pressure. [0003] At present, the commonly used methods for analyzing albumin are: (1) dry test strip detection method; using the protein error principle of the indicator (that is, the indicator ion is combined with the opposite charge carried by the albumin, so the pH color displayed by the reaction is displayed. to a higher pH color change, the magnitude of this pH color change being proportional to the albumin content). (2) Heating acetic acid method; heating and boiling to denature and coagulate the protein, an...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/76
Inventor 邢达徐未周静
Owner SOUTH CHINA NORMAL UNIVERSITY
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