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Maize autocopulation strain assistant selection and breeding method and application thereof

An inbred line, corn technology, applied in the directions of application, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of limited selection, long breeding cycle, large impact, etc., to achieve simple operation, shortening Breeding cycle, good repeatability

Inactive Publication Date: 2007-03-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limits the selection of traits
Moreover, the traditional method requires at least 6 to 8 selfing generations for the selection of basic materials, so the breeding cycle is relatively long and requires more human and financial resources.
And it is greatly affected by the breeder's subjective factors and objective environment

Method used

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  • Maize autocopulation strain assistant selection and breeding method and application thereof
  • Maize autocopulation strain assistant selection and breeding method and application thereof
  • Maize autocopulation strain assistant selection and breeding method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Breeding of maize inbred lines based on single cross species Shendan 16

[0032] 1. Screen the pair of Shendan 16S 0 SSR primers with polymorphism in generation population

[0033] (1) Extract the genomic DNA of the female and male parents of Shendan 16

[0034] According to the CTAB method, the genomic DNA of the female parent and the male parent of Shendan 16 were extracted respectively, and the specific methods are as follows:

[0035] 1. Take the female parent-inbred line K12 (National Crop Germplasm Preservation Center) and male parent-inbred line Shen 137 (National Crop Germplasm Preservation Center) of Shendan 16 respectively. ML centrifuge tube, then put it into liquid nitrogen to pre-cool.

[0036] 2. Take out the centrifuge tube in liquid nitrogen and quickly grind the blades in the centrifuge tube until it is ground into powder.

[0037] 3. Add CTAB buffer (1.17M NaCl, 0.016M EDTA PH=8.0, 0.0835M Tris PH=7.5, 2% CTAB, 1% β-mercaptoethanol) to the centr...

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PUM

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Abstract

The invention relates to a method for breeding corn cross system. Wherein, it comprises (1), selecting at least 20 SSR primers with polymorphism on the basic material S0 generation; each isopathic couple of chromosomes has at least two SSR primers whose genetic distance is at least 50cm; (2), using at least six couples of SSR primers, selecting single one of S1 generation that purified combined on said six SSR primers, while SSR primers are at least at six couples of isopathic chromosomes; (3), using at least 14 SSR primers expect from the ones in step (20, selecting S2 generation, to obtain the S2 single one purified combined on said 14 SSR primers, as the core cross system.

Description

Technical field [0001] The invention relates to a method for assisting the selection and breeding of maize inbred lines and its application. Background technique [0002] Maize (Zea mays) is one of the most widely cultivated crops in the world today. Maize has the characteristics of high yield, large yield potential, strong adaptability, and rich nutrition. In addition to being directly used as feed and grain, corn is also an indispensable raw material for many industries such as medicine, food, papermaking, chemical industry, and brewing, and plays an important role in the national economy. As a natural cross-pollinated crop, the heterozygosity of its genetic basis and the resulting breeding program that uses the heterosis of the F1 generation between inbred lines have formed the particularity of maize breeding. [0003] Maize inbred lines are self-progeny of homozygous genotypes and uniform traits separated from maize varieties or various hybrids through continuous selfing and ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 王建华王国英孙振耀
Owner CHINA AGRI UNIV
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