Penicillium fungus with nematocidal activity and its preparing method and use

A technology for killing nematodes and fungi, which is applied in the field of fungi that poison and kill plant-parasitic nematodes and their preparation, can solve the problems of poor selectivity, destruction of soil biota, large waste of chemical nematicides and the like

Inactive Publication Date: 2007-04-11
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has poor selectivity, strong extinction, and damages soil biota. Zhang Keqin, He Shichuan, Zhou Wei, etc. 1991. The role and research progress of fungi and bacteria in nematode biocontrol. Insecticidal Microbes. 3: 55-66), the 21st century is called the environmental protection century, some effective chemical nematicides Therefore, the research on the biological control of plant parasitic nematodes has naturally become a hot issue.

Method used

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  • Penicillium fungus with nematocidal activity and its preparing method and use
  • Penicillium fungus with nematocidal activity and its preparing method and use
  • Penicillium fungus with nematocidal activity and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Cultivation of strain P. pinophilum snf44-1

[0018] The mycelium of P. pinophilumsnf44-1 was inoculated on the test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; distilled water 1000mL. Incubate at 25°C for 10 days to obtain test tube seeds.

[0019] The test tube is inoculated into 250mL Erlenmeyer flask (100mL of every bottle) liquid culture medium again, and the culture medium formula is (weight percentage): glucose (2.83%), NH 4 Cl (0.15%), K 2 HPO 4 (0.12%), MgSO 4 ·7H 2 O(0.08%), KCl(0.02%), FeSO 4 (0.02%), the rest is distilled water, pH 6.5. At 25°C to 28°C, the shaker speed is 150r·min -1 ~200r·min -1 , fermentation 240h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.

Embodiment 2

[0020] Example 2: Cultivation of strain P. pinophilum snf44-1

[0021] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0022] The liquid medium formula is: glucose (2%), NH 4 Cl (0.1%), K 2 HPO 4 (0.08%), MgSO 4 ·7H 2 O(0.04%), KCl(0.02%), FeSO 4 (0.002%), pH 7.

Embodiment 3

[0023] Example 3: Cultivation of strain P. pinophilum snf44-1

[0024] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0025] The liquid medium formula is: glucose (3%), NH 4 Cl (0.15%), K 2 HPO 4 (2%), MgSO 4 ·7H 2 O(0.1%), KCl(0.2%), FeSO 4 (0.004%), pH 6.

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Abstract

The present invention belongs to the field of biological pesticide technology, and is especially one fungus strain with nematocidal effect and its culturing method, metabolic product and application. The fungus strain is Penicillium pinophilum snf44-1 in the preservation number of CGMCC No. 1852. It is prepared through liquid fermentation culturing, and its metabolite has obvious poisoning effect on plant nematode, especially on Meloidogyne hapla. The present invention has excellent application and development foreground.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00
Inventor 段玉玺马希斌陈立杰
Owner SHENYANG AGRI UNIV
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