Method of controlling plant disease damage by using bacillus and controlling agent

A plant disease control, Bacillus genus technology, applied in the directions of botanical equipment and methods, plant growth regulators, bacteria, etc., can solve the problems of increased drug resistance and dependence of pathogenic microorganisms

Inactive Publication Date: 2007-04-11
株式会社树
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, disease control relies heavily on the use of pesticides, which is detrimental to the environment, society, and agriculture globally, such as residual pesticides in soil, residual pesticides in food, and increased resistance of pathogenic microorganisms
Also, the overuse of pesticides is a safety issue for farmers

Method used

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  • Method of controlling plant disease damage by using bacillus and controlling agent
  • Method of controlling plant disease damage by using bacillus and controlling agent
  • Method of controlling plant disease damage by using bacillus and controlling agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Determination of the maximum growth time of BAL bacteria

[0084] A strain related to Bacillus amyloliquefaciens (hereinafter referred to as "BAL bacteria") (one platinum circulation) was suspended in 9 ml sterile water, and 100 μl of the resulting suspension was added to 100 ml of AG medium (1% glucose (Wako Pure Chemical Industries, Ltd.), 1% polyptone (Nihon Pharmaceutical Co., Ltd.), 0.1% KH 2 PO 4 (Wako Pure Chemical Industries, Ltd.) and 0.05% MgSO 4 ·7H 2 O (Wako Pure Chemical Industries, Ltd.), pH 7.00, autoclaved for 15 minutes) in a 300ml Erlenmeyer flask or 100ml potato infusion liquid medium containing 2% sucrose. (Potato dip is prepared as follows: Cut 200g potatoes into about 1cm 3 , add about 1 liter of distilled water and boil it on low heat for 30-40 minutes, then filter through double gauze. 2% sucrose was added to the filtrate and distilled water was added until the volume reached 1 liter. The resulting product is called "PS medium" and placed ...

Embodiment 2

[0088] Inhibitory Effect of BAL on the Proliferation of Xanthomonas campestris Pathogenic Variety

[0089] Scrape a loop of BAL bacteria from the YPA slant medium and inoculate it into a 300ml Erlenmeyer flask containing 100ml of AG medium. The obtained product was shaken and cultured at 120 rpm at 25° C. for 48 hours with a reciprocating shaker with an amplitude of 10 cm. Xanthomonas campestris pv. campestris suspension (100 μl) was added to the culture solution, and cultured with shaking under the same conditions for 48 hours. The suspension of Xanthomonas campestris pv. campestris used herein is prepared as follows: scrape a ring amount of culture on the slant medium of potato steep agar medium (hereinafter referred to as "PSA") containing 2% sucrose campestris pv. campestris was suspended in 9 ml sterile water. As a comparative experiment (control group), 100 μl of suspension of Xanthomonas campestris pathogenic variety campestris was added to 100 ml of AG medium without...

Embodiment 3

[0093] Preparation of seeds artificially contaminated with Xanthomonas campestris pathogenic variety

[0094] Pakchoi seeds (34 g, variety: Fuyushoumi) were wrapped with gauze and soaked in 500 ml of a 150-fold dilution of Chemicron G (neutral calcium hypochlorite; available chlorine: 70%) for 10 minutes in order to sterilize the seeds. After the seeds were sterilized, they were rinsed under running water for 1 hour, then dehydrated and dehydrated overnight at 35°C. In addition, Xanthomonas campestris pv. campestris was cultured on 5 PSA plates for 3 days in advance, and all the growing bacteria were scraped and suspended in 100ml distilled water to prepare 1×10 12cfu / ml Xanthomonas campestris pv. campestris bacteria liquid. Artificially contaminated seeds were prepared by soaking sterilized seeds (17 g) in a solution of Xanthomonas campestris pv campestris for 20 minutes and then dehydrating at 35°C overnight. Checked the degree of infection and found that the number of inf...

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Abstract

It is intended to provide a controlling agent or a controlling material for biologically controlling plant disease damage caused by a phytopathogenic bacterium, a fungus or a virus; a method of controlling plant disease damage by using the same; and a bacterium usable therein. Namely, a controlling agent or a controlling material for plant disease damage containing a bacterium which belongs to the genus Bacillus and is capable of inhibiting the infection with phytopathogenic bacterium, a fungus or a virus or growth thereof; a method of applying the same to a plant; and a bacterium usable therein.

Description

technical field [0001] The invention relates to a control method for bacterial, fungal or viral plant diseases and a control agent or material thereof. Background technique [0002] In the agricultural cultivation of commercial crops such as vegetables, the development of seed-borne, uploaded or air-borne or water-borne plant diseases usually involves many aspects such as the production of high-quality crops, stable yields and profitability. Especially when faced with abnormal weather conditions prone to plant diseases, commercial farms can be devastated. [0003] The main pathogens considered to be the causative agents of this class of transmissible diseases are filamentous fungi, bacteria and viruses. This is essential in order to achieve normal cultivation and prevent and control plant diseases caused by said pathogens. [0004] To make agricultural products effective against diseases, chemical techniques are widely used, such as the application of insecticides such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 油木大树木曾皓
Owner 株式会社树
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