Method for preparing inter-adhesive multilayered bacteria cellulose membrane

A technology of bacterial cellulose film and thin film, applied in medical science, bandages, absorbent pads, etc., can solve problems such as unsatisfactory effects

Inactive Publication Date: 2007-05-23
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current bacterial cellulose membranes are all single-layer, and the application effect is not very ideal; therefore, we hereby invent a multi-layer bacterial cellulose membrane

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Take 0.2ml of liquid culture medium with a sterile pipette, drop it into the ampoule containing Acetobacter xylinum strain, oscillate gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the suspension of the bacteria, and transplant it on a slope for culture cultured at 33°C for 1 day, transferred to the same slant medium, and transferred once every other day, and repeated 6 times to complete the activation process of the strain; inoculate a ring of activated slant Put into the seed medium, stir and cultivate at 25°C under a magnetic stirrer with a rotating speed of 400rpm / min for 20h, then insert 4% inoculum into the liquid medium and cultivate for 3 days; (2) carefully observe the surface of the liquid medium, and When a layer of film is formed on the surface, slowly add the re-prepared culture solution to the film with a dropper, so that the average depth of its distribution is 3mm; (3) repeat the operation (2) every 5 days; take out t...

Embodiment 2

[0024] (1) Use a sterile pipette to draw 0.6ml of liquid culture medium, drop it into the ampoule containing the Glucobacterium strain, oscillate gently to dissolve the freeze-dried bacteria into a suspension, absorb all the suspension of the bacteria, and transplant it on the slant medium cultured at 25°C for 3 days, transferred to the same slant medium, and transferred once every 3 days, and repeated 10 times to complete the activation process of the strain; insert a ring of activated slant Seed culture medium was stirred and cultivated at 30°C for 22 hours under a magnetic stirrer with a rotation speed of 420rpm / min, and then inserted into the liquid medium with 6% inoculum size and cultivated for 3 days; (2) carefully observe the surface of the liquid medium, on the surface When a layer of film is formed, slowly add the re-prepared culture solution to the film with a dropper, so that the average depth of distribution is 4mm; (3) Repeat operation (2) every 4 days, repeat 2 t...

Embodiment 3

[0029] (1) Use a sterile pipette to draw 0.4ml of liquid medium, drop it into the ampoule tube rich in Acetobacter strains, shake gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the suspension of the bacteria, and transplant it on the slant medium cultured at 30°C for 2 days, transferred to the same slant culture medium, transferred once every 2 days, and repeated 8 times in this way to complete the activation process of the strain; insert a ring of activated slant strain Seed culture medium was stirred and cultivated at 28°C for 24 hours under a magnetic stirrer with a rotation speed of 400rpm / min, and then inserted into the liquid medium with 8% inoculum size and cultivated for 3 days; (2) carefully observe the surface of the liquid medium, on the surface When a layer of film is formed, use a dropper to slowly add the re-prepared culture solution to the film so that the average depth of distribution is 5mm; (3) repeat the operation (2) every 3...

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Abstract

The invention relates to a method for preparing adhesive multilayer bacterial cellulose film, which comprises (1), activating bacterial, and planting activated slope bacterial into seed cultivate substrate, at 25-33Deg. C, mixing in magnetic mixer at 400-450rpm/min for 20-25h; planting into liquid cultivate substrate to be cultivated for 6-10 days; (2), when the surface has one layer of film, using drop pipe to add cultivate fluid into film; (3), repeating step (2) each 5-6 days, until the film depth is 3-8mm; (4), quickly separating and purifying cellulose film, to be stored in refrigerator. The inventive film can be used in medical skin, etc.

Description

technical field [0001] The invention relates to a preparation method of bacterial cellulose film, in particular to a preparation method of multilayer bacterial cellulose film adhered to each other. Background technique [0002] Cellulose is the main component of natural plant fiber. Bacterial cellulose is a type of cellulose produced by microorganisms. From the molecular composition of cellulose, both are composed of β-D-glucose through β-1,4-glucoside The straight chains formed by bonds are parallel to each other, not in a helical conformation, and have no branched structure, also known as β-1,4-glucose. Bacterial cellulose and plant cellulose are chemically the same, but bacterial cellulose, as a new type of biological material, has many unique properties. (1) High Young's modulus, high tensile strength and excellent shape retention. (2) Ultrafine (nanoscale), the formation process of the bacterial fiber ribbon is as follows: the diameter of the cellulose microfibril sec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44
Inventor 王华平孙晓玉邹瑜陈仕艳颜志勇石帅科
Owner DONGHUA UNIV
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