Four conjunction diagnostic kit of antimyocardial antibody

A diagnostic kit and myocardial antibody technology, which can be used in material analysis, measuring devices, instruments and other directions by observing the impact on chemical indicators, can solve the problems of lack of specificity and difficulty in clinical promotion of endomyocardial biopsy.

Inactive Publication Date: 2007-05-23
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Endomyocardial biopsy lacks specificity

Method used

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  • Four conjunction diagnostic kit of antimyocardial antibody

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Preparation of kit detection reagents

[0094] 1. Synthesis of Antigenic Peptides

[0095] Antigenic peptides with the same antigenicity as the four myocardial antigens were synthesized.

[0096] 1. The four myocardial antigen peptides and their amino acid sequences are:

[0097] ④ Cardiomyocyte mitochondrial inner membrane ADP / ATP carrier protein antigenic peptide (ANT);

[0098] ⑤β 1 Adrenergic receptor antigen peptide (β 1 );

[0099] ⑥ M 2 - Cholinergic receptor antigenic peptides (M 2 );

[0100] ④ Myosin heavy chain antigen peptide (MHC).

[0101] Amino acid sequence of ADP / ATP carrier protein peptide:

[0102] ANT-1 Pro-Ile-Glu-Arg-Val-Lys-Leu-Leu-Leu-Gln

[0103] ANT-2 Tyr-Asp-Glu-Ile-Lys-Lys-Phe-Val

[0104] beta 1 Amino acid sequence of adrenergic receptor peptide:

[0105] His-Trp-Trp-Arg-Ala-Glu-Ser-Asp-Glu-Ala-Arg-Arg-Cys-Tyr-Asn-Asp-Pro-Lys-Cys-Cys-Asp-Phe-Val-The-Asn- Arg-Cys

[0106] m 2 -Amino acid sequence of cholinergic receptor peptid...

Embodiment 2

[0141] Operation procedure using the diagnostic kit of the present invention

[0142] 1. Prepare washing liquid: take an appropriate amount of concentrated washing liquid and dilute it with distilled water according to the ratio of 1:9 (volume ratio) for later use;

[0143] 2. Add sample diluent: add 100 μl to each reaction well with a sampler;

[0144] 3. Add samples and leave blank control: Add 1 μl of each sample to be tested into each well of the existing sample diluent, and leave one well without adding a sample as a blank control.

[0145] 4. Add negative control: Add negative control serum to the 2 wells set as negative control, add 1 μl to each well;

[0146] 5. Mix well and incubate in a 37°C incubator for 60 minutes;

[0147] 6. Wash the plate: Pour off the contents, fill the reaction well with washing solution, let it stand for 3 minutes, pat dry and repeat this 3 times;

[0148] 7. Add enzyme-labeled secondary antibody: add 100 μl enzyme-labeled secondary antibo...

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Abstract

The invention provides a anti-myocardial antibody four-linking diagnostic reagent kit, including the coated reaction plate packaged the same antigen atomic manpower synthetic peptide antigen with four myocardial antigens, horseradish peroxidase labeled goat anti-human IgG ELISA two-antibody, sample dilution solution, enrichment solution, color reagent A, color reagent B, negative corresponding serum, termination solution, and brochure. The reagent kit of the invention has high sensitivity and strong specificity for viral myocarditis and dilated cardiomyopathy.

Description

technical field [0001] The invention relates to a medical detection kit, in particular to a myocardial antibody detection kit. Background technique [0002] Viral heart disease (VHD) is a group of heart diseases associated with viral infection, common viral myocarditis (VMC) and dilated cardiomyopathy (DCM) [see Kandolf R, Klingel K, Zell R, et al. Molecular mechanisms in the pathogenisis of enteroviral heart disease: acute and persistent infections. Clin Immuno Immunopathol, 1993, 68(2): 153]. [0003] In recent years, the incidence of VHD has gradually increased. Myocarditis occurs in about 5% of patients during the epidemic period of viral infection, of which 12.5% ​​can be transformed into DCM, while the incidence of DCM in the general population is only 8-10 / 100,000 population [see Liu Ying, Liao Yuhua, Tu Yuanshu, Ke Significance of Saatchi virus infection and anti-ADP / ATP carrier antibody in the pathogenesis of viral heart disease. J Clin Cardiovascular, 1997;13:14...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/78
Inventor 廖玉华王敏汪朝晖苑海涛董继华王朝晖袁瑾程翔
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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