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Sword-leaved cymbidium leaf virus spot enzyme-linked assay kit, preparation thereof and detecting method

A detection kit and a technology for building orchid leaves are applied in the preparation of test samples, measuring devices, and material analysis by observing the effect on chemical indicators, so as to achieve a simple preparation method, easy storage, and high repeatability. Effect

Inactive Publication Date: 2007-06-06
厦门华侨亚热带植物引种园
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AI Technical Summary

Problems solved by technology

At present, there is no literature report on the use of dot immunoassay for the detection of Jianlan mosaic virus and the product of the dot ELISA detection kit for the detection of the virus

Method used

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Experimental program
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Embodiment Construction

[0050] Main reagents used in the present invention: Jianlan mosaic virus polyclonal antibody is prepared by Xiamen Overseas Chinese Subtropical Plant Introduction Garden; PEG6000 (polyethylene glycol 6000), 2-mercaptoethanol, Triton-100 (Triton X-100), bovine Serum albumin (BSA) was purchased from Sigma company; goat anti-rabbit enzyme-labeled antibody, BCIP, NBT, DEAE ion exchange packing, IgG affinity chromatography packing were purchased from Pierce company; other conventional drugs and reagents used in the test All reagents were domestic analytical grade reagents.

[0051] 1. Reagent kit

[0052] Jianlan mosaic virus spot enzyme-linked detection kit is mainly composed of a box body, various bottled liquids and PVDF membranes (polyvinylidene fluoride membrane) located in the box body. Various bottled liquids include:

[0053] Insulation solution I 1 bottle

[0054] Insulation solution II 1 bottle

[0055] 1 bottle of color development buffer

[0056] 1 color developer ...

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Abstract

The invention discloses double-antibody sandwich ELSA regent box and the method for the Cymbidiummosaicvirus spot. The box is made up of the box body, every using liquid and the PVDF film. The preparation of the regent box is simple, quick, safe and reliable. The sample can be used little and it can be detected in a little time. Comparing to other serology detecting method, the method is simple, quick, sensitivity and high specialty, also it does not need the special device and get the result by the eye directly. So it can be used widely in the base unit.

Description

technical field [0001] The invention relates to a kit for detecting Jianlan mosaic virus and a preparation and detection method thereof, in particular to a Jianlan mosaic virus spot enzyme-linked detection kit and a method for preparing the kit and using the kit for Jianlan mosaic virus spot enzyme detection method of detection. Background technique [0002] Cymbidium mosaic virus (Cymbidium mosaic virus, CyMV) belongs to the genus Potato virus, which can cause chlorotic spots and necrotic spots in Jian orchid, local necrosis in Cattleya, and mosaic leaves in Cymbidium. In addition, it can also infect tropical spice crops herbs Orchid is one of the main viruses that endanger various ornamental orchids. In view of the harmfulness of CyMV to my country's orchid industry, it is very important to study its detection technology. [0003] The enzyme-linked immunosorbent assay (ELISA) method is commonly used for plant virus detection. Enzyme immunoassay is a technology that combi...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N21/78G01N1/34G01N33/531
Inventor 明艳林郑国华童庆宣李梅陈良华
Owner 厦门华侨亚热带植物引种园
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