Armillaria luteo-virens and its culture process and application

A yellow-green Armillaria and culture method technology, applied in the field of extracellular active polysaccharide, to achieve the effects of accelerating growth rate, reducing artificial culture and increasing yield

Inactive Publication Date: 2007-06-27
ZHEJIANG UNIV
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Problems solved by technology

Preservation unit: General Microbiology Center of C

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Screening of Armillaria Chrysanthemum ZJUQH

[0013] Isolation was screened from the Qinghai-Tibet Plateau region, and Armillaria armillaria in the soil area where Gastrodia elata was planted was mainly used as the isolated sample. The isolated samples were cultured by the single-spore method, and a single colony was picked on the primary screening medium. After cultivating for an appropriate time, the colonies showing blue on the primary screening medium were picked out for re-screening. A bacterial strain with faster growth rate and more secretion and accumulation of exopolysaccharide obtained from fermentation re-screening is numbered as a natural isolated strain, and its mycelium biomass reaches 2g / min after cultured at 23°C, natural pH and 120r / min for 5 days. L, the accumulation of exopolysaccharide was 3.5g / L.

[0014] After microwave and ultraviolet combined mutagenesis, the mycelium biomass and exopolysaccharide secretion reached 5g / L and 5.4-6g / L respectively...

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Abstract

The present invention discloses a kind of Armillaria luteo-virens ZJUQH CGMCC No.1884. Armillaria luteo-virens is cultured in Ca's culture medium. It has flat pileus with inwards rolled edge, medium-sized fruiting body in 5-12 cm width, white or yellowish thick flesh, dense and wide fungus plaits, white or yellow cylindrical stipe of 2-8 cm length and 2-2.5 cm diameter, and annulus in the middle position. The present invention provides new way for developing new edible and medicinal fungus and metabolite.

Description

Technical field: [0001] The invention relates to the field of bioengineering, in particular to cultivating plateau rare edible fungi at high density using bioengineering technology, and using the cultivating technology to obtain high-yield extracellular active polysaccharides. Background technique: [0002] Edible and medicinal fungi are a general term for a class of fungi that are both edible and medicinal, and fungal polysaccharides mainly refer to monosaccharides with more than ten molecules connected by glycosidic bonds that exist in the mycelium cell wall or are secreted outside the cell. made polymers. [0003] Edible and medicinal fungi have a long history in my country as medicine. As early as the end of the Eastern Han Dynasty more than 2,000 years ago, the world's first medicinal monograph "Shen Nong's Materia Medica" recorded the medicinal effects of fungi such as Ganoderma lucidum, Huoquan, and pigs; Li Shizhen, a famous medical scientist in the Ming Dynasty, wr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04
Inventor 陈启和
Owner ZHEJIANG UNIV
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