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Method of making liposomes, liposome compositions made by the methods, and methods of using the same

a technology of liposome composition and composition, applied in the field of making liposome composition, can solve the problems of other limitations of the related art, and achieve the effects of preventing oxidative stress or damage, and high dose of liposome composition

Active Publication Date: 2018-07-10
LIVON LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method for making liposomes with an entrapped agent, which can be used for various therapeutic purposes. The method involves dissolving lipid molecules in water to form a lipid solution, and then mixing it with an agent to be entrapped. The resulting solution can be injected into another solution or allowed to hydrate for a suitable period of time. The resulting liposomes have a specific size and contain a specific amount of lipids and agent. The liposome composition can be used to treat various conditions such as scurvy, cardiovascular disease, and cancer. The patent also describes the use of different agents such as ascorbic acid, GSH, and ALA. The patent also provides methods for administering the liposome composition to a subject in high doses.

Problems solved by technology

Other limitations of the related art will become apparent to those of skill in the art upon a reading of the specification.

Method used

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  • Method of making liposomes, liposome compositions made by the methods, and methods of using the same
  • Method of making liposomes, liposome compositions made by the methods, and methods of using the same
  • Method of making liposomes, liposome compositions made by the methods, and methods of using the same

Examples

Experimental program
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Effect test

example 1

Preparation of Sodium Ascorbate Entrapped Liposomes

[0141]97 g Phospholipon 50IP (a non-GMO vesicle-forming phosphatidylcholine lipid available from Lipoid) was solubilized in 56.3 g 200 proof alcohol at room temperature. Care was taken to ensure that all lipids were completely solubilized and no undissolved lipid remained. 109,5 g sodium ascorbate and 26.4 mg EDTA was separately solubilized in 251 g reverse osmosis-treated water at room temperature with normal agitation. The pH of the Na ascorbate-EDTA solution was adjusted to 6.3 The sodium ascorbate-EDTA solution was filtered with a 0.2 μm filter to remove dust particles and other contaminants. Streams of the lipid solution were slowly injected into the vitamin solution with continuous vortexing with a mixer. After all the lipid solution was added, lipid hydration was allowed to continue about 15 minutes with frequent mixing. At this point, the liposomes had formed in a smooth, translucent fluid.

[0142]2.2 g xanthan gum (available ...

example 2

Comparison of Empirical Liposomes and LivOn Liposomes

[0143]Several batches of Lypo-Spheric™ Vitamin C liposomes were obtained from LivOn Laboratories. According to the product ingredient table, each 5.7 mL packet of Lypo-Spheric™ Vitamin C liposomes contains 1,000 mg of sodium ascorbate and 1.0 g of essential phospholipids. The Lypo-Spheric™ Vitamin C liposomal product ranged in color from light orange to brownish. A second commercial product, Liposomal Vitamin C liposomes, were obtained from Empirical Laboratories. According to the product ingredients table, each 4 mL serving size of Empirical Liposomal Vitamin C liposomes contains 1000 mg sodium ascorbate and 400 mg of phosphatidylcholine.

[0144]The LivOn and Empirical liposome formulations were compared for sodium ascorbate amounts, particle size, amount of dry material and amount of lipid (estimated) with the results shown in Table 2. The Empirical liposomes appeared dilute in comparison to the LivOn liposomes. The Empirical lipo...

example 3

Comparison of Cold Process Liposomes to LivOn Laboratories Liposomes

[0152]Cold process liposomes were prepared essentially as described in Example 1, Lypo-Spheric™ Vitamin C liposomes were obtained from LivOn Laboratories. Various properties of the liposome formulations were determined with the results shown in Table 3.

[0153]The weight percent of ascorbic acid in the liposome formulations was determined by HPLC. The alcohol content was determined by gas chromatography. Mean particle size was determined by dynamic light scattering. Sodium ascorbate encapsulation was determined by filtration and HPLC as described in Example 2.

[0154]

TABLE 3Comparison of Cold Process Liposomes and LivOn LiposomesCold ProcessLivOn LaboratoriesLiposomesLypo-Spheric ™ Vitamin CVitamin C (ascorbic19.6%16.5%acid) %Phospholipids (wt %)18.8%18.7%Alcohol 10-11% 9-10%Mean particle size300-450 800-1200(nm)Particle size150-200600-900distribution SD (nm)Sodium ascorbate3535encapsulation (%)pH6.36.0-6.5Vitamin C wt ...

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Abstract

A method of preparing liposomes, liposome compositions formed by the process, and methods of using the liposome composition are provided herein.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This applications claims the benefit of provisional application Ser. No. 61 / 430,024 filed Jan. 5, 2011, incorporated by reference herein in.TECHNICAL FIELD[0002]The subject matter described herein relates to methods for preparing liposomes, which comprise an active agent or desired compounds in liposome-entrapped form, and to methods of using the liposome compositions.BACKGROUND[0003]Liposomes are spherical, self-enclosed vesicles composed of amphipathic lipids. They have been widely studied and have been used as vehicles for in vivo administration of agents, compositions, and compounds. A liposome comprises at least one closed lipid bilayer membrane which defines an aqueous compartment. Liposomes have long been used for drug delivery by encapsulating water soluble agents within the internal aqueous compartment and / or water insoluble agents within the lipid bilayer. Liposomes may be unilamellar, having one lipid bilayer membrane, or multi...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K9/127A61K31/198A61K31/375A61K31/385
CPCA61K31/375A61K31/385A61K31/198A61K9/127A61P3/00A61P3/02A61P3/10A61P7/04A61P9/00A61P19/06A61P27/02A61P27/12A61P35/00A61P39/02
Inventor ZHANG, YUANPENG
Owner LIVON LAB
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