Trichoderma spp strains with high capacity of fungus biological control and selection process thereof by molecular markers

a technology of biological control and trichoderma spp, which is applied in the field of trichoderma spp strains with high capacity of fungus biological control and selection process thereof, can solve the problems of not being able to carry out a largely effective and productive biological control, not all isolated microorganisms present optimal characteristics for their use in biological control, and not being able to use them in the best manner, so as to achieve the effect of high capacity for fungus biological control

Inactive Publication Date: 2004-05-20
COSMOCEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

0009] An object of the present invention is to provide Trichoderma spp. strains with high capacity for fungus biological control in wide ranges of temperature and pH.

Problems solved by technology

However, not all isolated microorganisms present optimal characteristics for its use in biological control.
However, up to date it has not been possible to carry out a largely effective and productive biological control, since no selection from suitable isolate or strain at country and weather conditions prevailing at the place where they are to be used has been carried out, so as according to the pathogen(s) agent(s)-object.
The fact that no strain nor isolate can be considered of universal utility for control of all kinds of pathogens and all kinds of environmental conditions, makes that, despite the great development the use of these microorganisms have had in biological control, up to date it has not been possible to use them in the best manner, since in some cases the growth of the control agent is not enough, or basically it can not control all present pathogenic agents.
Furthermore, when mixtures of strains or Trichoderma species are used to try to control different biologically pathogens, in some cases they inhibit each other creating a poorer biological control, instead of the improvement desired.

Method used

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  • Trichoderma spp strains with high capacity of fungus biological control and selection process thereof by molecular markers
  • Trichoderma spp strains with high capacity of fungus biological control and selection process thereof by molecular markers
  • Trichoderma spp strains with high capacity of fungus biological control and selection process thereof by molecular markers

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Embodiment Construction

[0039] A collection of seventy seven Trichoderma spp. strains identified as CIEAV-1 and CIEAV-77 was subjected to step 1 of growth rates measurement, which was done by growing the strains in Petri dishes with potato-dextrose-agar at pH conditions of 5, 6, 7, 8 y 9 at a temperature of 27.degree. C. Likewise, the seventy seven strains were growing at a pH of 5.6 at temperatures of 20, 27 y 37.degree. C. Once the growing were accomplished, 32 strains that display the best average growth rates were identified.

[0040] The 32 identified strains were subjected to step 2 of molecular-genetic markers detection step, by an AFLP analysis from which dendograms of genetic distance were constructed. For this, primers EcoRI 5'AGACTGCGTACCAATTC-3' (SEQ ID NO:1) and Msel 5'GACGATGAGTCCTGAGTAA-3' (SEQ ID NO:2) were used with an additional nucleotide (EcoRI+A ans Msel+A) for pre-amplification. Likewise, to obtain a suitable number of additional bands for analysis, primers with two additional selective ...

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Abstract

Trichoderma spp. strains with high capacity for fungus biological control in wide ranges of temperature and pH are described, such strains being compatible with each other. Likewise, a process of selection of such strains through molecular markers is described. The described process reduce the number of necessary experiments to determine if a Trichoderma strain, not previously described, can display a biological activity more acceptable than those well known.

Description

[0001] The present invention refers to techniques for fungus biological control and, more specifically, it refers to Trichoderma spp. strains with high capacity of fungus biological control and a process for selection thereof by molecular markers.[0002] Survival of agricultural cultivates during its development, in the face of multiple pathogenic agents attacking them, is due principally to the natural existence of biological control. Several microorganisms capable of antagonizing the effect of other phytopathogenic microorganisms give this control.[0003] Development and application of this natural potential is becoming more and more important as man has become able to participate and improve it and, will almost certainly have a big impact in agriculture in the near future, since the use of biological control agents in the country is an important factor in reducing chemical pesticides and therefore pollution, caused thereby.[0004] In general, the microorganisms used in biological co...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/38C12Q1/18
CPCA01N63/04G01N2333/37C12R1/885C12Q1/18A01N63/38C12R2001/885C12N1/145
Inventor HERRERA-ESTRELLA, ALFREDO HERIBERTOGALDAMES-GUTIERREZ, RAFAEL EDUARDOMARTINEZ-HERNANDEZ, JOSE PEDRO
Owner COSMOCEL
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