Light activated gene transduction for cell targeted gene delivery in the spinal column

Inactive Publication Date: 2005-03-10
RUBERY PAUL T +1
View PDF18 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016] A feature of preferred embodiments of the present invention the ability to overcome the problems involved with using traditional UV and γ-irradiation, by using locally administered UV, preferably long wavelength UV light, in order to induce the target cells to more effectively stimulate the transduction of a UV activa

Problems solved by technology

This aging or degenerating, which is incompletely understood, generally results in decreased biomechanical shock absorption, increased range of motion and pain and/or disability.
The introduction of either the co-infection or the DNA damaging agents dramatically induces the rate limiting step of second strand synthesis, i.e. the second strand of DNA which is synthesized based on the vector inserted first strand.
However, making use of these DNA damaging agents is impractical because the administration of an adenovir

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Light activated gene transduction for cell targeted gene delivery in the spinal column
  • Light activated gene transduction for cell targeted gene delivery in the spinal column
  • Light activated gene transduction for cell targeted gene delivery in the spinal column

Examples

Experimental program
Comparison scheme
Effect test

Example

[0056] The results of a completed proof of principle experiment are shown in Example 1.

EXAMPLE 1

[0057] I. Methods

[0058] A. Isolation of Human Mesenchymal Stem Cells

[0059] Human Mesenchymal Stem Cells (hMSC) were isolated from patient blood samples harvested from the iliac crest. The blood samples were diluted in an equal volume of sterile Phosphate Buffered Saline (PBS). The diluted sample was then gently layered over 10 ml of Lymphoprep (Media Prep) in a 50 ml conical tube (Corning). The samples were then centrifuged at 1800 rpm for 30 minutes. This isolation protocol is a standard laboratory technique, and the resulting gradient that formed enabled the isolation of the hMSCs from the layer immediately above the Lymphoprep. The isolated fraction was placed into a new 50 ml conical tube, along with an additional 20 ml of sterile PBS. The sample was centrifuged at 1400 rpm for 8 minutes. The supernatant was removed the cell pellet was resuspended in 20 ml for fresh PBS, and centr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Wavelengthaaaaaaaaaa
Wavelengthaaaaaaaaaa
Wavelengthaaaaaaaaaa
Login to view more

Abstract

In accordance with the present invention, methods and structures are provided for the treatment of functional spinal unit injuries through the use of light activated gene therapy to induce bone fusion through the introduction of a desired gene into a patient's spinal tissue. Methods and structures are also provided for the utilization of ultraviolet light activated gene therapy to repair/rebuild an injured intervertebral disc through the introduction of a desired gene into a patient's spinal tissue. An implant system including a light probe and an implant with which r-AAV is integrated is also provided.

Description

RELATED APPLICATION [0001] This application is a divisional of U.S. application Ser. No. 10 / 357,273, filed Jan. 31, 2003, which is incorporated herein by reference in its entirety.GOVERNMENT INTEREST [0002] This invention was made with Government support under NIH Contract #AR45972, an RO1 grant awarded by NIAMS. The Government has certain rights in the invention.BACKGROUND OF THE INVENTION [0003] 1. Field of the Invention [0004] The present invention relates to the field of gene therapy. According to the present invention, methods are provided for the treatment of functional spinal unit injuries through the use of light activated gene therapy to introduce a desired gene into a patient's tissue. An embodiment of the present invention includes methods for the utilization of light activated gene therapy to repair / rebuild an injured intervertebral disc. Alternate embodiment provide an implant system having UV activated viral vector integrated with an implant. [0005] 2. Description of t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K48/00C12N15/864
CPCA61K48/00A61K48/0008C12N2750/14143C12N15/86A61K48/0083
Inventor RUBERY, PAUL T.SCHWARZ, EDWARD M.
Owner RUBERY PAUL T
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap