Microfluidic devices for electrophoretic analysis of materials
Inactive Publication Date: 2005-09-01
CAPLIPER LIFE SCI INC
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[0007] The present invention provides for a microfluidic system for high-speed electrophoretic analysis of subject materials for applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other areas. The system has a channel in a substrate, a light source and a photoreceptor. The channel holds subject materials in solution in an electric field so that the materials move through the channel and separate into bands according to species. The light source excites fluoresc
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While these operations are fast compared to macroscale electrophoretic methods, the operations fall short of a hi
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[0014] General Description of Microfluidic Systems
[0015]FIG. 1 discloses a representative diagram of an exemplary microfluidic system 100 according to the present invention. As shown, the overall device 100 is fabricated in a planar substrate 102. Suitable substrate materials are generally selected based upon their compatibility with the conditions present in the particular operation to be performed by the device. Such conditions can include extremes of pH, temperature, salt concentration, and application of electrical fields. Additionally, substrate materials are also selected for their inertness to critical components of an analysis or synthesis to be carried out by the system.
[0016] Useful substrate materials include, e.g., glass, quartz and silicon, as well as polymeric substrates, e.g., plastics. In the case of conductive or semiconductive substrates, there should be an insulating layer on the substrate. This is particularly important where the device incorporates electrical ...
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Abstract
The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 09 / 977,056 filed Oct. 12, 2001, which is a continuation of U.S. patent application Ser. No. 09 / 378,169 filed Aug. 19, 1999 (now U.S. Pat. No. 6,337,740), which is a continuation of U.S. patent application Ser. No. 09 / 207,864 filed Dec. 8, 1998 (now U.S. Pat. No. 6,233,048), which is a continuation of U.S. patent application Ser. No. 08 / 941,679 filed Sep. 30, 1997 (now U.S. Pat. No. 5,852,495), which is a continuation of U.S. patent application Ser. No. 08 / 683,080 filed Jul. 16, 1996 (now U.S. Pat. No. 5,699,157).BACKGROUND OF THE INVENTION [0002] There has been a growing interest in the manufacture and use of microfluidic systems for the acquisition of chemical and biochemical information. Techniques commonly associated with the semiconductor electronics industry, such as photolithography, wet chemical etching, etc., are used in the fabrication of these microfluidic ...
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