Rabbit skin comprising biological active substances and its use

a biological active substance and rabbit skin technology, applied in the field of rabbit skin, can solve the problems of not having a method to prepare rabbit skin, and achieve the effects of inhibiting the activity of anti-complements, promoting the activation of macrophages, and inhibiting the activity of 48-hour homologous pca reaction

Inactive Publication Date: 2006-03-09
VANWORLD PHARMA (RUGAO) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010] The rabbit skin of the present invention possesses 0.5 iu / g SART activity or more, and which also possesses the kallikrein-protease inhibition activity.
[0011] By extracting with organic solvent, processing with acid, processing with alkali, absorbing, eluting and concentrating, biologically active preparations being rich in amino acids and nucleic acids can be prepared from the rabbit skin. The said amino acids include glutamic acid, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, aspartic acid, threonine and serine. The said nucleic acids include urocanic acid, uracil, hypoxanthine, xanthine and thymine.
[0012] Drug can be prepared by combining the biologically active preparations of the present invention with pharmaceutical acceptable adjuvants. This drug can be various form of preparations used clinically, including injection and tablet, the preferred form is the injection. For injections, the adjuvants can be for example injectable distilled water, normal saline, injectable vegetable oil, glucose injection, propylene glycol, polyethylene glycol, or it may be all kinds of stabilizers and emulsifiers. For tablets, capsules and granules, the adjuvants can be excipients such as starch, lactose, mannitol; binders such as crystalline cellulose, arabic gum, corn starch, glutin, polyethylene, polyvinyl alcohol, polyvinyl-pyrrolidone; disintegrants such as carboxylmethyl cellulose, poly-ethylene glycol, potato starch; lubricants such as talcum powder, magnesium stearic acid; moistening agents such as glycerol. For ointments, the adjuvants can be fat oil, paraffin, wool fat, vaseline, glycol, glycerol.
[0013] The pharmacological and clinic experiments showed that the drugs prepared from the rabbit skin of the present invention have analgesic effect against all kinds of symptomatic neuralgia, lambago, cholecystagia, angina, arterial embolism pains, acute pains from wound, burn and scald, pains in surgery or post-surgery, peptic ulcer pain, dysmenorrhea, labor pains posterior to childbirth, headache, pains induced by various tumor and so on.
[0014] The study showed that the drugs prepared from the rabbit skin of the present invention can effectively promote activation of macrophage, significantly inhibit 48-hour homologous PCA reaction induced by antibody of IgE in the model of type I allergic reaction in mice, inhibit the activity of anti-complement in type II allergic reaction. The effects have linear correlation with the doses. So the drugs have effects on inhibiting inflammatory reaction correlated with immunity and improving immunity function.
[0015] Moreover, the drugs prepared from the rabbit skin of the present invention have anti-allergic, anti-ulcer and sedative effects and so on.

Problems solved by technology

There has not been any method to prepare rabbit skin that contains strongly active and high yield biologically active substances.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Preparation of Rabbit Skin

[0023] The dry variola vaccine of vaccinia virus Lister strain was dissolved by PBS(−) (NaCl 80 g, KCl 2 g, NaH2PO4 11.5 g, KH2PO4.2H2O 2 g, adding injectable H2O to 10 L) and well shaken. 0.4 ml of this solution was injected in central inner lamina of testicle of Japanese white rabbit. Dislocating its cervical vertebra on the fourth day. Cut the scrotum, removed connective tissue in the testicle. The testicle was placed in vessel full of ice, preserved at −80° C. refrigerator. The testicle was taken from refrigerator, softened for 1 h, grinded at 4° C., mixed in 1:1 with EAGLES' cultural medium (Eagle's powder 9.4 g, 10% NaHCO3 12.5˜22.0 ml, glutamine 10 ml, injectable H2O 1 L), packed, frozen at −80° C. in refrigerator for 1 h, thawed at 37° C. in water bath, centrifuged with 3500 rpm at 4° C. for 20 min, packed by 10 ml. The subculture antigen was preserved at −80° C. in refrigerator. The virus solution of subculture antigen was taken from the −8...

example 2

[0024] Preparation of Rabbit Skin

[0025] The vaccinia virus Ikeda strain and New Zealand white rabbit were used to prepare the subculture antigen according to example 1.

[0026] The virus solution of subculture antigen was taken from the −80° C. refrigerator, thawed at 30° C. in incubator. 5 ml of the virus solution was added into 500 ml of PBS(−) by a 10-ml syringe, well shaken and diluted to injection of 109 virus / ml. A New Zealand white rabbit weighing 2.75 kg with its back shaved, was sterilized with 75% ethanol, injected subcutaneously with the virus injection 0.3 ml per site in 250 sites with no leaking, no injecting without the virus injection and no puncturing throughout skin. The rabbit injected was fed for 3 days. When inflammatory tissue showed that skin surface had visible blains accompanying with changing colour from redness to mauveness and skin became thick, and subcuticle and hip became swollen, killed the rabbit by cervical vertebra dislocation and peeled in 15 min. ...

example 3

[0027] Preparation of Rabbit Skin

[0028] The vaccinia virus Dairen strain and Chinese rabbit were used to prepare the subculture antigen according to example 1.

[0029] The virus solution of subculture antigen was taken from the −80° C. refrigerator, thawed at 30° C. in incubator. 5 ml of the virus solution was added into 500 ml of PBS(−) by a 10-ml syringe, well shaken and diluted to injection of 106 virus / ml. A Chinese rabbit weighing 1.5 kg with its back shaved, was sterilized with 75% ethanol, injected subcutaneously with the virus injection 0.1 ml per site in 250 sites with no leaking, no injecting without the virus injection and no puncturing throughout skin. The rabbit injected was fed for 3 days. When inflammatory tissue showed that skin surface had visible blains accompanying with changing colour from redness to mauveness and skin became thick, and subcuticle and hip became swollen, killed the rabbit by cervical vertebra dislocation and peeled in 15 min. The skin of rabbit w...

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Abstract

A rabbit skin containing biologically active substances is obtained by the process including vaccinating rabbit skin tissues with vaccinia virus, feeding a rabbit vaccinated with vaccinia virus, killing the rabbit when its skin tissues are sufficiently inflamed, and skinning the rabbit. The rabbit skin of the present invention can be used for preparing drugs and health foods.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a rabbit skin containing biologically active substances and its use. BACKGROUND OF THE INVENTION [0002] It was reported that the extracts from inflammatory rabbit skin tissues vaccinated with vaccinia virus can be used for the treatment of allergic disease and have the analgesic effect. There has not been any method to prepare rabbit skin that contains strongly active and high yield biologically active substances.DETAILED DESCRIPTION OF THE INVENTION Aims [0003] The objective of the present invention is to provide a rabbit skin which is rich in biologically highly active substances that can be used for preparing drugs and health foods. Project [0004] As a result of many years of hard work, the inventors of the present invention have reached this aim. [0005] The rabbit skin of the present invention is obtained by the process including vaccinating rabbit (Oryctolagus cuniculus) skin tissues with vaccinia virus, feeding r...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/275A61K35/12A61K39/285A23J1/10A61K35/36A61K39/00A61P25/04A61P29/00
CPCA23J1/10A61K35/12A61K2039/54A61K39/285A61K35/36A61K39/12A61P17/02A61P25/04A61P25/20A61P29/00A61P35/02A61P37/08
Inventor CHEUNG, WING SUM
Owner VANWORLD PHARMA (RUGAO) CO LTD
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