Methods and compositions using Substance P to promote wound healing

a technology of substance p and composition, applied in drug compositions, peptides, metabolic disorders, etc., can solve problems such as trauma, surgical procedures, disease, etc., and achieve the effects of promoting healing, promoting cellular growth and activity, and enhancing growth and activity

Inactive Publication Date: 2007-07-05
AUXANO BIOLOGICS
View PDF29 Cites 55 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011] According to the present invention, neuropeptides, including but not limited to, tachykinins, calcitonin gene-related peptide, analogs thereof, and conjugates thereof are applied topically to wounds in mammalian tissue in order to promote healing. Such neuropeptides have been found to possess cellular growth promoting activity when administered alone to a wound in tissue and have been further found to provide enhanced growth promoting activity when combined with other cellular growth promotants, such as epidermal growth factor, transforming growth factor-β, insulin, insulin-like growth factor, nerve growth factor, and platelet derived growth factor. In addition, the neuropeptides have been found to promote the elaboration of cellular matrices and the development of cellular attachment mechanisms which enhance the regeneration of tissue having a substantially normal morphology.

Problems solved by technology

Such wounds may result from trauma, surgical procedures, and disease.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and compositions using Substance P to promote wound healing
  • Methods and compositions using Substance P to promote wound healing
  • Methods and compositions using Substance P to promote wound healing

Examples

Experimental program
Comparison scheme
Effect test

experimental examples

Example 1

Use of Substance P to Treat a Vascular Wound

[0079] Substance P exhibits properties which may be capable of stimulating wound healing. Based on this, the purpose of the following example was to evaluate the effect of topically applied substance P on wound healing in full thickness skin punch wounds in a rat model. In addition, selenocystamine was tested as a potential wound treatment at the same time.

Animal Model and General Care

[0080] Twenty-one male Sprague-Dawley rats weighing between 300-350 g were used in this study. The rats were housed in the temperature controlled vivarium at Texas Tech Health Science Center with a 12-hour alternating light cycle, and were provided water and rat chow ad libitum. Animals were allowed several days of adjustment upon arrival before the surgery to get rehydrated and familiar with the environment. The study protocol was approved by the Texas Tech University Health Sciences Center Animal Care and Use Committee which assured that the a...

example 2

Effect of SP±IGF-1 in Epithelial Healing after Photorefractive Keratectomy in Rabbits

[0092] Corneal wound healing after Photorefractive keratectomy and its consequences, epithelial defect and haze, have been studied extensively. Results on how the absence of corneal epithelium affects the stromal keratocytes are contradictory. Some studies showed that an early decrease in the density of keratocytes is followed by an increased number of these cells in the underlying stroma and polymorphonuclear (PMN) inflammatory reaction (Hirst et al., 1981; Kenyon et al., 1979). This stromal change is related to stromal haze and instability of the refractive result. Other studies show that a traumatic removal of the epithelium would prevent changes in the stromal healing (Harmann et al., 1962; Nakayasu et al., 1988).

[0093] Studies suggest that the epithelium influences the cellular activation and metabolic activities of stromal cells during wound healing (Dohlman, 1971; Johnson-Muller et al., 197...

example 3

Substance P Analogs

[0118] As mentioned previously, Substance P (SP) is a ubiquitous mammalian neuropeptide having the structure RPKPQQFFGLM (SEQ ID NO:1). In order to determine which portion of SP is responsible for its binding to the NK1 receptor, physiological assays have been performed using SP analogs that were systematically shortened, and it was found that the six amino acids at the carboxy terminal end of the molecule were required for most of SP's binding to its receptor SP6-11 (SEQ ID NO:8) showed activity of about 50-90% of that which was found for the whole SP molecule. Of the six C-terminal residues, the importance of the phenylalanine residues at positions 7 and 8 for the SP-NK1 receptor interaction was demonstrated by the finding that the substitution of another amino acid for either of these residues results in a dramatic change in reactivity. For example, replacement of either Phe7 or Phe8 by a D-phenylalanine residue results in a 20-fold decrease in the binding of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
molecular weightaaaaaaaaaa
depthaaaaaaaaaa
Login to view more

Abstract

Healing of wounds in mammalian tissue may be enhanced by the application of certain neuropeptides, optionally in combination with known growth promoting hormones. Exemplary neuropeptides include tachykinins, such as Substance P, Substance K, and the like, as well as calcitonin gene-related peptides. The compositions may further include a polymeric delivery carrier and are utilized by applying to the site of the wound. Wounds may be vascular or avascular wounds. The compositions promote elaboration of cellular matrices and development of cellular attachment mechanisms in addition to stimulating cellular proliferation.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] The present application claims benefit under 35 U.S.C. 119(e) of provisional applications U.S. Ser. No. 60 / 738,910, filed Nov. 22, 2005; U.S. Ser. No. 60 / 739,154, filed Nov. 22, 2005; and U.S. Ser. No. 60 / 740,489, filed Nov. 28, 2005. The contents of each of the above-referenced patent applications are hereby expressly incorporated herein by reference.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] Not applicable. BACKGROUND OF THE INVENTION [0003] 1. Field of the Invention [0004] The present invention relates generally to methods and compositions for the enhancement of cellular proliferation and for the treatment of wounds and other disorders. More specifically, the invention relates to the use of neuropeptides for wound treatment in general and corneal wound treatment and vascular wound treatment in particular and, more particularly, to the use of Substance P and analogs and conjugates thereof for such wound tr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/19A61K38/20A61K38/30A61K9/127A61K9/14
CPCA61K38/046A61K38/30A61L15/42A61L15/44A61L2300/25A61L2300/412A61L2300/414A61L2300/602A61L2300/45A61K2300/00A61P17/02
Inventor REID, TEDMURPHY, CHRISTOPHER J.
Owner AUXANO BIOLOGICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap