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Prevention of allergy in children

a technology for allergy prevention and children, applied in the field of allergy prevention in children, can solve the problems of ineffective measures, increased risk of hypersensitivity or inflammation, and unknown, and achieve the effect of reducing the chance of affecting the intinal mucosa and prolonging the time span

Inactive Publication Date: 2008-02-07
FORSKARPATENT I SYD AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0023] According to the present invention human infants who, very early in life, have been exposed to staphylococcal superantigen in vivo by colonization of their intestines by toxin-producing S. aureus (S. aureus enterotoxin A, B, C or D, or TSST-1), are less prone to develop gastro-intestinal symptoms from food allergy and possibly also eczema by 18 months of age than all other infants, including those who are colonized by non-toxin-producing S. aureus at the same site. In another aspect of the invention, human infants who are colonized by toxin-producing S. aureus in the first few weeks of life have more Tregs in their blood circulation by 4 months of age. Furthermore, their blood cells respond with less production of IL-5 and IL-13 than other infants in response to stimulation with birch pollen antigen, including those who are colonized by non-toxin-producing S. aureus at the same site. This suggests that they may have lesser risk of developing hypersensitivity and hay fever to birch pollen later in life. Also human infants who are colonized by toxin-producing S. aureus in the first few weeks of life have less T-cells with the surface marker CD29 in their blood circulation by 4 months of age. This suggests that they may have a lesser risk of developing inflammatory reactions.

Problems solved by technology

However, these measures have been completely ineffective, in that only minute amounts of antigen is needed to trigger IgE production.
However, it is not known which types of microbes are important for this to occur.
There is an endless variety of bacteria, viruses and parasites, some of which might be important in providing the right type of stimuli to the immune system, others which may be ineffective, or even increase the risk of developing hypersensitivity or inflammation.
This means that they bind to and activate a large proportion (10-30%) of the T-cells in human beings or animals, resulting in a massive cytokine production that may lead to shock and severe symptoms, even death.
It has, thus, been suggested that toxins elaborated by S. aureus can worsen the reaction by stimulating T-cells, leading to tissue damage.
However a treatment that results in anergy or deletion of T-cells would not be recommended as prevention for allergy in children since decreased T-cell function would lead to a poor defense against infections.
It has been described that the number and function of CD25+ Treg can be increased by in vitro stimulation with polyclonal activators as well as specific antigens and transfer of these antigen-expanded cells into mice results in delayed development of autoimmune disease in susceptible mice.
This observed activation / reduction together with the fact that superantigen in the blood circulation leads to shock in humans are the main reasons why Staphyloccocal enterotoxins administered into the blood are unsuitable for treatment of humans.
However, it is completely unethical and impossible to do such procedures as preventive measures in small children.

Method used

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Examples

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example 1

Characterization of CD25+ Tregs

[0040] The development of CD25+ Tregs during the first 4 months of life were characterized by analysing the number of cells expressing surface CD4 and CD25 and intracellular CTLA-4 in peripheral blood obtained at 4 months of age compared to cord blood. FIG. 1A shows a dot-plot of the expression of CD25 and CTLA-4 on gated CD4+ T-cells. The cells in the upper right quadrant (A) were presumed to be CD25+ Tregs.

[0041] However, CD25 may also be upregulated on T-cells during activation, and whereas the average number of CD25+ Tregs was similar in newborns and children 4 months of age, the number of CD4+CD25+ CTLA-4− increased with age, indicating that they represented activated T-cells. This is seen in FIG. 1B, which shows the number of CD25+ Tregs; CD4+CD25+CTLA-4+ and CD4+CD25+CTLA-4− cells×10−6 per ml blood, respectively, in newborns and children at 4 months of age. CD4+CD25+ CTLA-4− cells are cells that recently have been activated, expressing CD25 on...

example 2

Intestinal Colonization by Toxin-Producing S. aureus Induces Expansion of Cd25+ Tregs

[0043] The type of colonization pattern was examined in relation to the degree of CD25+ Treg expansion. FIG. 2A shows the frequency of colonization with S. aureus at 3 days, 1, 2, 4, and 8 weeks, in the groups of children above and below the median ratio of CD25+ Tregs. We observed that the children who had a CD25+ Treg ratio above the median were significantly more frequently colonized by S. aureus at 3 days and at 4 weeks than the children with a CD25+ Treg ratio below the Median. This was not observed for any other bacterial group or species (data not shown).

[0044] In FIG. 2B it is shown the S. aureus colonization pattern of infants belonging to each of the quartile with respect to CD25+ Treg expansion. A ‘dose-response’ pattern was observed with the 4th quartile showing the most rapid acquisition. In this group, 45% of the children were colonized by S. aureus by day 3 and 91% by 4 weeks. None ...

example 3

S. aureus does not Induce a General Activation of T-Cells

[0046] Apart from being a marker of CD25+ Tregs, CD25 is also expressed by activated T-cells. FIG. 3A shows Ratio (4 months / cord blood) of number of CD4+CD25+CTLA-4− T-cells (cells that recently have been activated, expressing CD25 on the surface, but without the intracellular expression of CTLA-4. These cells are not able to suppress helper T-cell functions). In order to exclude that S. aureus induces activation of T-cells rather than generation of CD25+ Tregs, the colonization frequency in children was compared with a ratio of the numbers of CD4+CD25+CTLA-4− T-cells above and below the median (2.03). We observed no difference in S. aureus colonization between the groups of children at 3 days, 1, 2, 4, and 8 weeks, above and below the median, neither for total S. aureus (FIG. 3B) nor for toxin-producing S. aureus (FIG. 3C). These results support that toxin-producing S. aureus induce an increase in CD25+ Treg counts and not o...

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Abstract

The present invention relates to a use of bacterial superantigens in the manufacture of a pharmaceutical composition for mucous membrane administration for the prevention of inflammatory disorders in newborn infants, such pharmaceutical compositions, as well as method for prevention of inflammatory disorders.

Description

TECHNICAL FIELD [0001] The invention refers to the use of a bacterial superantigen for administration onto the mucous membrane in newborn infants for the prevention of allergies, autoimmune and inflammatory disorders. BACKGROUND OF THE INVENTION [0002] A number of diseases are characterized by an exaggerated or untoward immune reactivity against harmless antigens. Such diseases include allergies, autoimmune diseases and inflammatory bowel diseases. Normally, immune responses to harmless antigens are suppressed, a mechanism called tolerance. Tolerance to specific antigens, either exogenous or endogenous, may be induced either by mucosal or systemic exposure. Tolerance occurs because helper T-cells are deleted, paralyzed or suppressed by other T-cells, so called regulatory T-cells. Allergies [0003] Allergies are defined as enhanced immune reactivity to one or several harmless environmental antigens, so called allergens. In IgE-mediated allergies, the allergic individual mounts an IgE...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/085A61K
CPCA61K39/085A61K2039/541A61K2039/55A61K2039/543A61K2039/542A61P37/08
Inventor ALDERBERTH, INGEGERDRUDIN, ANNAWOLD, AGNES E.
Owner FORSKARPATENT I SYD AB
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