Anthrax carbohydrates, synthesis and uses thereof

a technology of anthrax carbohydrates and synthesis, applied in the field of anthrax carbohydrates, synthesis, can solve problems such as the death of the hos

Inactive Publication Date: 2009-10-01
UNIV OF GEORGIA RES FOUND INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0045]The terms “comprises” and variations thereof do not have a limiting meaning where these terms appear in the description and claims. Unless otherwise specified, “a,”“an,”“the,” and “at least one” are used interchangeably and mean one or more than one.

Problems solved by technology

When spores of B. anthracis are inhaled or ingested they may germinate and establish populations of vegetative cells which release anthrax toxins often resulting in the death of the host.

Method used

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  • Anthrax carbohydrates, synthesis and uses thereof
  • Anthrax carbohydrates, synthesis and uses thereof
  • Anthrax carbohydrates, synthesis and uses thereof

Examples

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example 1

Cell Wall Carbohydrate Compositions of Strains from the B. cereus Group of Species Correlate with Phylogenetic Relatedness

[0146]The Bacillus cereus group of bacteria is comprised of the closely related species B. cereus, B. anthracis, and B. thuringiensis. Bacillus cereus strains can be potent opportunistic pathogens, while B. thuringiensis is an insect pathogen, and B. anthracis is the causative organism of anthrax. The distribution of B. anthracis spores in the US mail system in 2001 demonstrated their potential as a bioterrorist weapon. Although differentiation amongst B. cereus, B. thuringiensis and B. anthracis in practice is not difficult, the speed and specificity of confirmatory identification of virulent B. anthracis are of great importance in the context of bioterrorism preparedness and emergency response. In particular, there is a need for diagnostic tools based on the immunological response since this response has potential for great clinical sensitivity by virtue of amp...

example 2

The Structure of the Cell Wall Polysaccharide of Bacillus Anthracis is Species Specific

[0176]Bacillus anthracis contains only a few known carbohydrates as part of its vegetative cell wall and spore. In this example the structure of the polysaccharide released from the cell wall of the vegetative cell by aqueous hydrogen fluoride (HF) is described. This HF-released polysaccharide (HF-PS) was isolated and structurally characterized from the Ames, Sterne and Pasteur strains of B. anthracis. The HF-PSs were also isolated from the closely related B. cereus ATCC 10987 strain, and from the B. cereus ATCC 14579 type strain and compared to those of B. anthracis. The structure of the B. anthracis HF-PS was determined by glycosyl composition and linkage analyses, matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS), and one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. The HF-PSs from all of the B. anthracis isolates had an identical structure ...

example 3

Synthesis and Antigenic Analysis of the BclA Glycoprotein Oligosaccharide from the Bacillus anthracis Exosporium

[0216]The glycoprotein BclA is an important constituent of the exosporium of B. anthracis. This glycoprotein is substituted with an oligosaccharide composed of a 1,2-linked β-L-rhamnoside substituted with the previously unknown terminal saccharide, 2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-D-glucopyranose, also referred to as anthrose. Anthrose has not been found in spores of B. cereus and B. thuringiensis, making it a potential species-specific marker for B. anthracis. In order to study the antigenicity anthrose, efficient syntheses of an anthrose-containing trisaccharide and a series of structurally related analogs were developed. The analogs lacked either the methyl ether at C-2 or contained modified C-4 amino functionalities of anthrose. The synthetic compounds were equipped with an aminopropyl spacer to facilitate conjugation to the carrier proteins mcKHL ...

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Abstract

The present invention presents the isolation, characterization and synthesis of oligosaccharides of Bacillus anthracis. Also presented are antibodies that bind to such saccharide moieties and various methods of use for such saccharide moieties and antibodies.

Description

[0001]This application is a continuation-in-part of International Application No. PCT / US2007 / 015196, filed Jun. 29, 2007, which claims the benefit of U.S. Provisional Application Ser. No. 60 / 817,929, filed Jun. 30, 2006, and U.S. Provisional Application Ser. No. 60 / 933,937, filed Jun. 8, 2007; further this application claims the benefit of U.S. Provisional Application Ser. No. 61 / 056,204, filed May 27, 2008, and U.S. Provisional Application Ser. No. 61 / 132,515, filed Jun. 19, 2008; all of which are incorporated herein by reference in their entireties.GOVERNMENT FUNDING[0002]The present invention was made with government support under Grant Nos. AI 056061, R21 AI 059577, and GM 065248 warded by the National Institutes Health, and Grant No. DE-FG09-93ER20097, awarded by the Department of Energy. The Government has certain rights in this invention.BACKGROUND[0003]Bacillus anthracis is a gram-positive, spore-forming bacterium that causes anthrax in humans and other mammals. Because of t...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/395A61K39/00A61K39/385C12Q1/00G01N33/53C07K16/00C07H1/00
CPCA61K39/07A61K2039/55583A61K2039/6068C07H3/04C07H3/06G01N2469/00C07H9/04C07K16/1278G01N33/56911G01N2333/32G01N2400/00C07H5/04
Inventor CARLSON, RUSSELL W.BOONS, GEERT-JANBUSKAS, THERESECHOUDHURY, BISWAKANNENBERG, ELMARLEOFF, CHRISTINEMEHTA, ALOKSAILE, ELKERAUVOLFOVA, JANAQUINN, CONRADWILKINS, PATRICIAVASAN, MAHALAKSHMIWOLFERT, MARGREET A.
Owner UNIV OF GEORGIA RES FOUND INC
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