Water extract of antrodia camphorata for immunostimulatory effect and preparation method thereof

Inactive Publication Date: 2010-09-09
KAOHSIUNG MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]The water extract of the fruiting body of wild A. camphorata (abbreviated as ACW) is obtained by extracting the fruiting body of AC with water, and ACW can effectively induce immune response and stimulate DCs. ACW is further sequentially extracted with ethyl aceta

Problems solved by technology

Therefore, it is uneasily to find out the wide fruiting body of AC or identify the morphological appearance of this Aphyllophorales fungus.
In addition, the price of AC is still high due to their biologically active components having potential pharmaceutical value.
The fruiting body of AC cannot be easily harvested and be artificially cultured.

Method used

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  • Water extract of antrodia camphorata for immunostimulatory effect and preparation method thereof
  • Water extract of antrodia camphorata for immunostimulatory effect and preparation method thereof
  • Water extract of antrodia camphorata for immunostimulatory effect and preparation method thereof

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Experimental program
Comparison scheme
Effect test

experiment 2

action and Fractionation of ACW

[0046]ACW (216.4 mg) was extracted with solvents (sequentially as ethyl acetate, ethanol and water) of increasing polarities to obtain three different extracts, 8.9% (19.3 mg) of ethyl acetate extract (ACW-EA), 38.5% (83.3 mg) of ethanol extract (ACW-E) and 52.6% (113.8 mg) of a second water extract (ACW-W). Percentage is referred to the weight percentage of ACW, and weight is referred to the dry material extracted. The amount of carbohydrate in each extract was determined using phenol-sulfuric acid method (Chen et al., 2007), and the spectrum characteristics therein were analyzed with NMR.

experiment 3

[0047]The above-mentioned extracts were solved in deuterium solvents such as dideuterium monoxide (D2O), pyridine-D5 (C5D5N) and so on, and then 1H NMR spectra were recorded on a Varian Unity Inova-600 MHz NMR. Chemical shift was reported in parts per million (ppm, d).

experiment 4

of Human T Cells and DCs

[0048]The preparation method of DCs was mainly prepared and modified from Lin et al, 2006. First, peripheral blood mononuclear cells (PBMCs) were obtained from 12 healthy donors by centrifugation with the Ficoll-Hypaque (Amersham Bioscience, Uppsala, Sweden) density gradient centrifugation. PBMCs were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum, 1 mM L-glutamin, 100 μg / ml streptomycin and 100 U / ml penicillin for 2 hours. The nonadherent cells were removed, and the T cells were purified by nylon wool separation, as the source of mononuclear T lymphocytes. T cells were loaded into the column and the eluted nonadherent cell fraction was rich in T cells. As determined by FACS, the resulting cell population was 80% T cells with a high expression of CD3. The adherent cells were cultured for 6 days in RPMI 1640 medium with 50 ng / ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Half of the culture mediu...

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Abstract

A preparation method for a water extract of the fruiting body of Antrodia camphorata (ACW) is provided. The method includes steps of: (a) providing the fruiting body; and (b) boiling the fruiting body in water to obtain the water extract. This polysaccharide-rich water extract from A. camphorata induces the maturation of dendritic cells, enhances T cell proliferation and INF-γ production, and polarizes them toward the Th1 pathway. ACW can be effectively applied in cancer immunotherapy.

Description

FIELD OF THE INVENTION[0001]The present invention relates to an extract of a fruiting body of Antrodia camphorata and the preparing method thereof. In particular, the present invention relates to a water extract of a fruiting body of A. camphorata (ACW) and the preparing method thereof, and the water extract has immunostimulatory effect on dendritic cells (DCs).BACKGROUND OF THE INVENTION[0002]Antrodia camphorata (AC) is an endemic mushroom in Taiwan and grows in the internal heartwood (or the dark / humid wood surface) of the particular Cinnamomum kanehirai in 400 to 2000 meters altitude. Therefore, it is uneasily to find out the wide fruiting body of AC or identify the morphological appearance of this Aphyllophorales fungus. In addition, the price of AC is still high due to their biologically active components having potential pharmaceutical value.[0003]The fruiting body of AC cannot be easily harvested and be artificially cultured. In addition, mycelia products of AC are popular in...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCA61K39/39C12N2500/74C12N5/0639A61K2039/60A61P37/04A61K36/07
Inventor WU, YANG-CHANGLU, MEI-CHINCHANG, FANG-RONGDU, YING-CHIWU, TUNG-YING
Owner KAOHSIUNG MEDICAL UNIVERSITY
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