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Compositions and methods for augmentation and regeneration of living tissue in a subject

a technology of living tissue and composition, applied in the field of composition and methods for augmentation and regeneration of living tissue in subjects, can solve the problems of expensive procedures and extensive medical procedures

Inactive Publication Date: 2010-12-23
COLOPLAST AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a composition for regenerating and augmenting living tissue in a subject. The composition includes microparticles made of a biodegradable polymer and mammalian cells. The microparticles can be administered into the tissue to be regenerated or augmented, where they can help to promote tissue regeneration and recovery. The invention also provides methods for preparing and using the composition, as well as treating diseases and cartilage defects using the composition. The technical effects of the invention include improved tissue regeneration and augmentation, as well as potential treatment for related diseases and bone defects.

Problems solved by technology

Such procedures are costly, and require extensive medical procedures such as rehabilitation and physical therapy.
Methods using scaffold technologies of various forms, where the scaffold (with, or without cells grown in the scaffold) is inserted into the defect, have suffered from difficulties in performing the cell implantation procedure solely guided by arthroscopy.

Method used

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  • Compositions and methods for augmentation and regeneration of living tissue in a subject
  • Compositions and methods for augmentation and regeneration of living tissue in a subject
  • Compositions and methods for augmentation and regeneration of living tissue in a subject

Examples

Experimental program
Comparison scheme
Effect test

example 1

Equipment

[0349]Sonics 20 kHz ultrasonic probe

[0350]Sonotek 25 kHz ultrasonic probe

[0351]NE-1000 syringe pump, New Era Pump Systems, Inc.

[0352]10, 25, 50 mL Hamilton syringes

[0353]Polymer solution led to ultrasonic probe by 1 / 16″ PTFE-tubing

[0354]Polymer: MPEG-PLGA 2-30 kDa

[0355]Malvern Mastersizer 2000 with Hydro 2000s accessory for measuring suspensions.

[0356]Determination of particle size distribution: Particles were suspended in water with a small amount of sodium dodecyl sulfate and sonication and measured on the Malvern.

[0357]Optical miscroscopy: Olympus BX60, images were processed with Imagepro 5.1

experiment 1

[0358]A 4% (w / v) solution of polymer in acetone was atomized over a bath of stirred, cold (−50-(−30)° C.) isopropanol. The suspension was left to stir for 20 minutes and filtered cold with suction. The particles, were not sucked completely dry. They were dried in vacuum (0.04 mbar) for 24 h and stored in a closed vial until further characterization. FIG. 1 shows the optical microscopy, SEM and particle size distribution for experiment 1.

experiment 2

[0359]A 10% (w / v) solution of polymer in acetone was atomized and collected as described in expt. 1. FIG. 2 shows the optical microscopy, SEM and particle size distribution for experiment 2.

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Abstract

The present invention provides for a composition, for augmentation and regeneration of living tissue in a subject, comprising a population of porous microparticles of a biodegradable polymer, one or more mammalian cell populations, and optionally, a biocompatible adhesive.

Description

FIELD OF THE INVENTION[0001]The present invention relates to compositions and methods suitable for the augmentation and regeneration of living tissue in a subject.BACKGROUND OF THE INVENTION[0002]Tissue engineering methods using cell transplantation are known, and for example, may involve for instance open joint surgery (e.g., open knee surgery) and, in case of joint surgery, extensive periods of relative disability for the patient to recuperate in order to ensure that optimal results are achieved. Such procedures are costly, and require extensive medical procedures such as rehabilitation and physical therapy.[0003]Methods using scaffold technologies of various forms, where the scaffold (with, or without cells grown in the scaffold) is inserted into the defect, have suffered from difficulties in performing the cell implantation procedure solely guided by arthroscopy.[0004]Arthroscopic Autologous Cell Implantation (called AACI or ACI using minor surgical interventions) is a surgical ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/12A61K31/727A61K38/18A61K47/30A61K31/728A61K38/39A61P19/08A61P19/04C08G63/08C08G65/00C01B25/32A61K9/16B65D69/00
CPCA61L27/18A61L27/38A61L27/56Y10T428/2982A61L2400/06A61L27/3604C08L67/04A61P15/00A61P19/04A61P19/08
Inventor EVERLAND, HANNESAMUELSEN, PETERVANGE, JAKOBCLAUSEN, CHRISTIANGALLEGO, MONICA RAMOS
Owner COLOPLAST AS