Unlock instant, AI-driven research and patent intelligence for your innovation.

Polypeptide marker for diagnosis of arteriosclerosis, method for detection of arteriosclerosis by using the maker or the like, and kit for diagnosis of arteriosclerosis

a polypeptide marker and arteriosclerosis technology, applied in the field of polypeptide markers for diagnosis of arteriosclerosis, can solve problems such as unsatisfactory levels, and achieve the effect of high accuracy

Inactive Publication Date: 2011-11-24
FUJIKURA KASEI CO LTD +1
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides polypeptide markers, gene markers, antibodies, probes, DNA microarrays or chips, and methods for detecting arteriosclerosis using these markers. These markers can specifically detect an arteriosclerotic lesion and are useful for diagnosing arteriosclerosis. The invention also includes a kit for diagnosing arteriosclerosis. The use of these markers can help improve the diagnosis and treatment of arteriosclerosis.

Problems solved by technology

However, most of the detection markers of them are risk markers.
However, it does not satisfy an adequate level.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide marker for diagnosis of arteriosclerosis, method for detection of arteriosclerosis by using the maker or the like, and kit for diagnosis of arteriosclerosis

Examples

Experimental program
Comparison scheme
Effect test

examples

[0098]Hereunder is a detailed description of the present invention with reference to Examples. However, the present invention is not to be limited by the following description.

production example

Preparation of Samples

[0099](1) The subjects were patients with carotid artery stenoses who had visited our hospital or cooperative institutes. The reference (control) healthy subjects were selected from outpatients so that both groups had matched gender and age (±5 years). When they were visiting as outpatients or hospitalized in the neurosurgery department; 1) their families were explained that this study was to be conducted for research purposes and that cooperation with the study was not compulsory, and informed consents were obtained from them; 2) questions about the family history, the past history, the life style such as drinking and smoking habits, and the work style were asked; 3) blood was collected and separated into serum and corpuscle components and cryopreserved; and furthermore, 4) the clinical seriousness, the therapeutic process, the findings of the blood examination and the like were recorded on the basis of the medical record of the doctor. The patient blood as th...

example 1

Measurement of the Antibody Titer by ELISA Method

[0133]Using the experimental protocol of the Production Example mentioned above, the antibody levels of the thirteen out of the eighteen types of the detected arteriosclerosis diagnostic marker candidates were measured by ELISA, and the presence of the serum antibodies was confirmed. The results of the measurement are shown in Table 2.

TABLE 2Healthy Patient (P)subject (N)standardp valueStandardClone nameAveragedeviation(two-sided)AveragedeviationS19A30.3860.009120.00000.272 0.0624TS27A20.3060.1246 0.00110.229 0.0909S21B10.2060.1271 0.00780.146 0.0779S25E10.3460.3338 0.01450.210 0.1515TS12D20.3180.1959 0.02270.242 0.1019S36C30.0500.0407 0.02810.035 0.0207N48B10.2110.1109 0.04720.165 0.1065S28D10.4930.2525 0.06420.400 0.2251TS27H10.0930.0827 0.06450.067 0.0393PA1050.2560.1414 0.00000.14170.0891PA2020.2890.1546 0.00970.21570.1054PA2130.4400.2052 0.00020.29550.1368PA2340.1500.1001 0.01310.10460.0705

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
imaging testsaaaaaaaaaa
MRIaaaaaaaaaa
Login to View More

Abstract

Disclosed are: a polypeptide marker for diagnosing arteriosclerosis; a gene marker for diagnosing arteriosclerosis; an antibody; a probe for detecting an arteriosclerosis marker gene; a DNA microarray or a DNA chip for detecting an arteriosclerosis marker gene; a method for detecting arteriosclerosis; and a kit for diagnosing arteriosclerosis; with which an arteriosclerotic lesion can be detected with much improved accuracy. Specifically disclosed are: a polypeptide marker for diagnosing arteriosclerosis, which comprises a polypeptide having an amino acid sequence set forth in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, and 35 of the Sequence Listing, or a partial amino acid sequence thereof; a gene which encodes the amino acid sequence; a probe for detecting the gene; a DNA microarray or a DNA chip comprising the probe; an antibody bindable to the polypeptide as an antigen; a kit comprising any one of the above-mentioned items; and a method for detecting arteriosclerosis by using any one of the above-mentioned items.

Description

TECHNICAL FIELD[0001]The present invention relates to polypeptide markers for diagnosing arteriosclerosis, gene markers for diagnosing arteriosclerosis, antibodies, probes for detecting an arteriosclerosis marker gene, a DNA microarray or a DNA chip for detecting the arteriosclerosis marker genes, a method for detecting arteriosclerosis, and a kit for diagnosing arteriosclerosis.[0002]Priority is claimed on Japanese Patent Application No. 2008-209210, filed Aug. 15, 2008, the content of which is incorporated herein by reference.BACKGROUND ART[0003]Arteriosclerosis is a disease frequently found in aorta, coronary arteries, cerebral arteries, and carotid arteries, being a main cause of myocardial infarction and cerebral infarction. At present, the presence of atherosclerosis is said to be crucial for the ground of the onset of ischemic organ diseases such as ischemic heart disease and cerebrovascular disorder, which are top leading causes of death. Arteriosclerotic lesions are pathomo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C40B30/04C40B40/06C07K14/435C12Q1/68C07K16/18C07H21/04G01N33/566
CPCC07K14/47C12Q1/6883G01N2800/323G01N33/6893C12Q2600/158G01N33/5302
Inventor NAKAMURA, RIKAKURODA, HIDEYUKITOMIYOSHI, GOHIWASA, TAKAKITAKIGUCHI, MASAKISAEKI, NAOKATSUMACHIDA, TOSHIO
Owner FUJIKURA KASEI CO LTD