Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells

a technology of epithelial cells and gene expression profiles, which is applied in the field of diagnosis and prognostic methods of lung disorders using gene expression profiles from nose epithelial cells, can solve the problems of serious lung disease risk to individuals, serious lung disease risk, and insignificant survival rate, and achieve the effect of less invasiv

Inactive Publication Date: 2012-07-26
TRUSTEES OF BOSTON UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0014]The present invention provides a much less invasive method for diagnosing lung dis

Problems solved by technology

Lung disorders represent a serious health problem in the modern society.
In addition to cigarette smoke, exposure to other air pollutants such as asbestos, and smog, pose a serious lung disease risk to individuals who have been exposed to such pollutants.
Unfortunately survival rates have not changed substantially over the past several decades.
This is largely because there are no effective methods for identifying smokers who are at highest risk for developing lung cancer and no effective tools for early diagnosis.
However, none of these methods provide a combination of both sensitivity and specificity need

Method used

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  • Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells
  • Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells
  • Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells

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example 1

[0170]In this study, we obtained nucleic acid samples (RNA / DNA) from nose epithelial cells. We also obtained nucleic acids from blood to provide one control. We used our findings in the PCT / US2006 / 014132 to compare the gene expression profile in the bronchial epithelial cells as disclosed in the PCT / US2006 / 014132 to the gene expression pattern discovered in this example from the nasal epithelial cells.

[0171]We have explored the concept that inhaled toxic substances create a epithelial cell “field of injury” that extends throughout the respiratory tract. We have developed the hypothesis that this “field of injury”, measured most recently in our laboratory with high density gene expression arrays, provides information about the degree of airway exposure to a toxin and the way in which an individual has responded to that toxin. Our studies have been focused on cigarette smoke, the major cause of lung cancer and of COPD, although it is likely that most inhaled toxins result in a change ...

example 2

[0183]A Comparison of the Genomic Response to Smoking in Buccal, Nasal and Airway Epithelium

[0184]Approximately 1.3 billion people smoke cigarettes worldwide which accounts for almost 5 million preventable deaths per year (1). Smoking is a significant risk factor for lung cancer, the leading cause of cancer-related death in the United States, and chronic obstructive pulmonary disease (COPD), the fourth leading cause of death overall. Approximately 90% of lung cancer can be attributed to cigarette smoking, yet only 10-15% of smokers actually develop this disease (2). Despite the well-established causal role of cigarette smoke in lung cancer and COPD, the molecular epidemiology explaining why only a minority of smokers develop them is still poorly understood.

[0185]Cigarette smoking has been found to induce a number of changes in both the upper and lower respiratory tract epithelia including cellular atypia (3, 4), aberrant gene expression, loss of heterozygosity (3, 5) and promoter hy...

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Abstract

The present invention provides methods for diagnosis and prognosis of lung cancer using expression analysis of one or more groups of genes, and a combination of expression analysis from a nasal epithelial cell sample. The methods of the invention provide far less invasive method with a superior detection accuracy for lung cancer when compared to any other currently available method for lung cancer diagnostic or prognosis. The invention also provides methods of diagnosis and prognosis of other lung diseases, such as lung cancer.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 12 / 940,840, filed en Nov. 5, 2010, which is a continuation of U.S. application Ser. No. 12 / 282,320, filed on Sep. 9, 2008, which is a national stage filing under 35 U.S.C. 371 of international Application PCT / US2007 / 006006, filed Mar. 8, 2007, which claims the benefit under 35 U.S.C. 119(e) from U.S. provisional application Ser. No. 60 / 280,552, filed on Mar. 9, 2006, the content of which is herein incorporated by reference in their entirety. International Application PCT / US2007 / 006006 was published under PCT Article 21(2) in English.GOVERNMENT SUPPORT[0002]The present invention was made, in part, by support from the National Institutes of Health grant No. HL077498. The United States Government has certain rights to the invention.BACKGROUND OF THE INVENTION[0003]1. Field of the Invention[0004]The present invention is directed to methods for diagnosing long diseases from nasal ...

Claims

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Application Information

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IPC IPC(8): C40B30/04
CPCG01N33/57423C12Q1/6886C12Q2600/158
Inventor BRODY, JEROME S.SPIRA, AVRUMBERMAN, JEFFREY S.O'REGAN, ANTHONY W.
Owner TRUSTEES OF BOSTON UNIV
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