Indane dimers for use in the treatment of autoimmune inflammatory disease
a technology of autoimmune inflammatory disease and dimers, which is applied in the field of compounds, can solve the problems of no longer recommended use of these drugs to maintain remission in cd, no benefit in maintaining remission, and systemic absorption, and achieve the effect of improving the availability of medicaments
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experiment 1
Compounds 2-6
Mice
[0289]Specific Pathogen-Free BALB / c mice, 6-8 weeks of age, were obtained from a commercial supplier (Harlan UK). Mice were fed irradiated diet and housed in individually ventilated cages (Tecniplast UK) under positive pressure.
DSS treatment
[0290]DSS (5%) was dissolved in drinking water. Compounds were administered orally at a dose of 10 mg / kg or 30 mg / kg on days 0-7, and mice were culled on day 8 or day 9, depending on the severity of the disease. The mice were checked each day for morbidity and the weight of individual mice was recorded. Induction of colitis was determined upon autopsy, length of colon and histology. Colons were recovered and stored at −20° C. for immunological analysis. All of the compounds and experimental groups are randomly alphabetically labelled. Throughout experiments all data recording was performed in a blind manner. The codes on boxes / groups were not broken until after the data was analysed i.e. boxes labelled A, B, C etc were identified...
experiment 2
Compounds 31 and 47.
1. Procedures
2.1 Animals and Caging
[0318]A total of 90 BDF 1 (H. Pylori-free, murine norovirus-free) male mice (Harlan Laboratories, UK) were used in the study. Animals were 8-10 weeks old on supply and used at 10-12 weeks of age. All mice were held in individually ventilated cages (IVCs) in an SPF (Specific Pathogen Free) barrier unit. The animals were identified by numbered cages and by ear punches.
2.2 Diet and Animal Welfare
[0319]The animals were fed Rat and Mouse Expanded diet from B & K. Both feed and water (from drinking bottles) were available ad libitum. There was a constant room temperature of 21±2° C. and a mean relative humidity of 55±10%. The day-night cycle was constant, with light and dark phases of 12 hours each. Animal health was monitored daily and cages were cleaned at regular intervals.
2.3 Groups, Dosages, Administration and Formulations
[0320]Mice were randomised into study groups. All the mice in any one cage received the same treatment and we...
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