Compounds, Compositions and Methods for Attracting and/or Arresting Bed Bugs

a technology applied in the field of compositions and compounds for attracting and/or arresting bed bugs, can solve problems such as a wide resurgen

Inactive Publication Date: 2016-11-03
SIMON FRASER UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the last two decades there has been a world-wide resurgence, with bed bugs becoming common urban pests (Boase 2001; Myles et al.

Method used

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  • Compounds, Compositions and Methods for Attracting and/or Arresting Bed Bugs

Examples

Experimental program
Comparison scheme
Effect test

example 1

Maintaining a Colony of Common Bed Bugs for Production of Exuviae and Use in Bioassays

[0039]A colony of common bed bugs was kept in an insectary at 22-24° C., ambient relative humidity, and a photoperiod of 10 hours dark to 14 hours light. To collect pheromone for extraction, isolation and identification, the colony was increased from 2,400 to 6,000 bugs and held at the higher level for 18 months.

[0040]Approximately 150 bed bugs were kept in each of 40 50-ml jars. Each jar was fitted with a piece of cardboard (2×2 cm) at the bottom and a strip (2×5 cm) of corrugated cardboard diagonally across the jar. The jar was covered with a plastic lid perforated with small holes for ventilation.

[0041]Each bed bug was allowed to feed once per month on a human volunteer. At 1,500 bed bugs per week for 30 months, this amounts to 180,000 individual feedings. Jars with bed bugs to be fed were covered with fine mesh and pressed against the volunteer's forearm so that the bed bugs could feed through ...

example 2

General Experimental Design to Investigate the Response of Bed Bugs to Test Stimuli

[0042]Bioassays were run in dual-choice olfactometers and in large Plexiglass arenas (FIG. 1). Dual-choice olfactometers consisted of two lateral Pyrex® glass Petri dishes, connected to a central dish (all dishes 3×9 cm inner diameter) via a Pyrex® glass tube (2.5 cm long×2 cm inner diameter). The dishes in this olfactometer mimic the natural still-air shelters in which bed bugs spend the day. Prior to the start of bioassays, a disc of paper towelling (9 cm diameter) was placed into each dish and a strip of paper towelling (2.4×0.6 cm) was inserted into the connecting glass tubing to provide traction for walking bed bugs. In addition, a piece of filter paper (2×3 cm; Whatman) was placed into each lateral dish and covered with a piece of cardboard (2×2 cm) as a refuge for bioassay insects.

[0043]Treatment and control stimuli were randomly assigned to each lateral dish. Olfactometers were enclosed in opa...

example 3

Evidence that Bed Bug Exuviae (Cuticle Shed During Moulting) Induce Arrestment of Foraging Bed Bugs: Effect of Number and Age of Exuviae Tested

[0045]Experiment 1 tested whether 50 exuviae of 5th instar nymphal bed bugs (1 exuvia=0.08 mg) induce arrestment of foraging 5th instar nymphs. Given a strong arrestment response of nymphs to 50 exuviae (see Table 1), follow-up Experiments 2-4 tested whether fewer numbers of exuviae would suffice to induce arrestment responses. Experiment 5 explored whether exuviae which were aged at room temperature for 2 months are still effective in inducing arrestment of bed bugs.

[0046]In Experiments 1-4, 50, 10, 2 or 1 exuviae were unexpectedly all equally effective in inducing arrestment of bed bugs (Table 1). Surprisingly, in Experiment 5, exuviae after 2 months of storage at room temperature still induced arrestment of bed bugs.

Table 1 illustrates the effect of number and age of exuviae on the response of bed bugs in the three-dish, dual-choice olfact...

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Abstract

Disclosed is a six-component composition of volatile and less-volatile pheromone components that can be used alone or in combination with other compounds to attract and / or arrest bed bugs of both sexes and all developmental stages. This blend can be used to bait traps that are effective in capturing bed bugs in infested premises or can be combined with a pesticide that is lethal to bed bugs.

Description

FIELD OF THE INVENTION[0001]This invention pertains to compounds, compositions and methods for attracting and / or arresting bed bugs.BACKGROUND OF THE INVENTION[0002]For many years, from the end of World War II until the final decade of the 20th century, much of the world was free from infestation by the common bed bug, Cimex lectularius (Heteroptera: Cimicidae). This is attributed to modern hygienic measures and the widespread use of DDT and other persistent insecticides (Potter 2012). However, in the last two decades there has been a world-wide resurgence, with bed bugs becoming common urban pests (Boase 2001; Myles et al. 2003; Jones 2004), and sometimes causing debilitating skin irritation and lesions (Ter Porten et al. 2005). This resurgence has renewed interest in detecting, monitoring and controlling bed bug infestations, particularly the development of effective compounds, compositions and methods for attracting and / or arresting bed bugs.SUMMARY OF THE INVENTION[0003]The inve...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N43/50A01N35/02A01N25/08A01N25/18A01M1/02A01N33/08A01N31/04A01N35/06A01N35/04A01M1/20A01N31/02A01N37/02
CPCA01N43/50A01N31/02A01N35/02A01N25/08A01N25/18A01N37/02A01M1/2094A01N31/04A01N35/06A01N35/04A01M1/2011A01M1/023A01N33/08Y02A50/30A01N41/12
Inventor GRIES, REGINE M.BRITTON, ROBERT A.HOLMES, MICHAEL T.GRIES, GERHARD J.
Owner SIMON FRASER UNIVERSITY
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