Device and method for detecting a target analyte
a technology for detecting devices and analytes, applied in the field of devices and methods for detecting targets, can solve the problems of inability to fully integrate, affordable, point-of-care or point-of-need diagnostic platforms, and inability to perform a full-scale, comprehensive, and comprehensive diagnostic platform, etc., and achieve the effect of improving the accuracy and reducing the cost of the actual test, and improving the accuracy of the detection process
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first embodiment
[0040]a device 20 for detecting a target analyte shown in FIG. 1 includes a capillary tube 22 extending from a first end 24 to a second end 26. In one embodiment as shown, the first end 24 is elevated above the second end 26 to create an inclined capillary tube 22 for transporting a sample fluid containing a targeted analyte and magnetic beads that have been functionalized to identify and bind to the target analyte. The sample can be any biological sample such as, but not limited to whole blood, serum, plasma, CSF, saliva, and milk. It should also be appreciated that the sample could also be any fluid that contains a targeted analyte (e.g., water, buffer). While the inclined capillary tube 22 is identified and illustrated as being inclined, it should be understood that this is simply an example of a capillary or channel with a force to drive liquid through the channel. In the example of an inclined capillary tube 22 or channel, the motive force to drive the liquids through is the ef...
fourth embodiment
[0053]The channels 354 each include an inclined capillary tube 322 extending from a first end 324 to a second end 326. More specifically, the first end 324 is elevated above the second end 326 and is coupled to the outlet 356 of the channel 354 for transporting the secondary fluid sample containing a sample. The capillary tubes 322 each include a porous matrix 358 impregnated with a label for a target analyte and disposed in the inclined capillary tube 322 at the first end 324 for labelling target analyte in the secondary fluid sample. Optionally, the label for the target analyte can be conjugated to a magnetic bead and a magnet can be used to capture and move the anlyte through the system. Such direct labelling is most advantageous when multiplexing as done in the fourth embodiment, as compared to other embodiments. Each label can be to different targets or to the same target in each capillary tube 322 depending on whether the intent is to process a sample for the presence of multi...
third embodiment
[0055]A method of operating the device is also provided. The method includes the step of introducing a sample fluid into a flow splitter 348. Next separating the sample fluid of a first volume containing a sample into a plurality of secondary fluid samples each having a volume less the first volume. The method proceeds by transporting each second fluid sample into one of a plurality of inclined capillary tubes 322. Then, the method includes the step of dissolving a label from a porous matrix 358 with the secondary fluid sample in each of the plurality of inclined capillary tubes 322 to label a target analyte in response to the target analyte being present. The labeled target analyte is trapped by the capture antibodies 346 and / or the magnet. The next step of the method is adding a developer solution to each of the plurality of inclined capillary tubes 322. The method proceeds by reacting each of the plurality of labelled secondary fluid samples and the developer solution and measuri...
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Abstract
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