Methods for improving the efficiency of simultaneous saccharification and fermentation reactions

Abstract

The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

Description

1. CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a Divisional of U.S. application Ser. No. 13 / 516610, filed Feb. 22, 2013, which a 371 of PCT / US10 / 61082, filed Dec. 17, 2010, which claims the benefit of U.S. Provisional Application No. 61 / 289,917, filed Dec. 23, 2009, all of which are incorporated by reference in their entirety.REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY[0002]The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named 20180914_NB31433USPCD_SequenceListing.txt created on Sep. 14, 2018 and having a size of 148 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety.2. FIELD OF THE DISCLOSURE[0003]The present disclosure is generally directed to methods and compositions for improving product yield from simultaneou...

AI Technical Summary

Benefits of technology

The patent text discusses the discovery of certain enzymes that can lead to the accumulation of byproducts that reduce the yield of fermentation products. The text describes the use of certain other enzymes that can reduce or prevent the accumulation of these byproducts and improve the yield of fermentation products. In simple terms, this patent aims to improve the efficiency of certain enzymes in simultaneous saccharification and fermentation reactions.

Problems solved by technology

Nonetheless, the typical SSF reaction can be exceedingly lengthy, lasting, for example, several days in order to achieve modest ethanol concentrations (see, e.g., Kadam et al., 2004, Biotechnol. Progr. 20(3):705).

In particular, it is discovered that certain β-xylosidases with a retaining mechanism of action, when used in SSF reactions, can result in rapid accumulation of alkyl-β-xylopyranoside byproducts that would lead to a reduced yield of fermentation products.

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