Method for the removal/purification of serum albumins and means for use in the method
a serum albumin and purification technology, applied in the field of method for removal/purification of serum albumin and methods for use, can solve the problems of less suitable as ligands
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Examples
Embodiment Construction
Immobilisation: The protein used in this study was a 7.1 kDa fragment of Streptococcal protein G containing the complete albumin binding domain, A3 (fragment SPG-ABD3, aa 254-299 in Kraulis et al., FEBS.letter 378 (1996) 190-194). Approximately 2 mg of the fragment was immobilised on a 1 ml NHS-activated column, HiTrap, according to the manufacturers instruction (Amersham Pharmacia Biotech AB, Uppsala, Sweden) (NHS=N-hydroxy-succinimide).
Chromatography conditions: All samples were adjusted to pH 7.0 before loading onto the column. A buffer consisting of 20 mM sodium phosphate and 150 mM sodium chloride, pH 7.0, was used for equilibration and washing after sample loading on the column (Buffer A).
Elution was performed by decreasing pH to 2.7 by applying a 20 mM citrate, 150 mM sodium chloride buffer, pH 2.7 (Buffer B).
Samples: Human serum albumin HSA (Sigma, St Louis, Mo, U.S.A.) 2 mg / ml in Buffer A, bovine serum albumin BSA (Sigma, St Louis, Mo, U.S.A.) 2 mg / ml in Buffer A and acid t...
PUM
Property | Measurement | Unit |
---|---|---|
temperature | aaaaa | aaaaa |
temperature | aaaaa | aaaaa |
ionic strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com