A method for synthesizing citric acid glyceride by immobilized lipase catalysis in a multistage fixed bed reactor is disclosed, belonging to the technical field of the synthesis of citric acid glyceride. In the invention, monoglyceride and citric acid are used as reaction substrates, organic solvents like anhydrous isopropanol, anhydrous isoamylol or anhydrous tertiary amyl alcohol are used as reaction media, the immobilized lipase is used as a catalyst, and esterification reaction is carried out in the multistage fixed bed reactor to obtain the product of the citric acid glyceride. The invention, which takes the multistage fixed bed as the reactor and is provided with a molecular sieve packed column at the back of the reactors at every stage, facilitates the substrates to contact with the catalyst sufficiently, and meanwhile, removes the water generated from the reaction, in addition, the molecular sieve packed column is regenerated and refreshed periodically which is advantageous for the esterification reaction. Prohibition effect against the esterification reaction can be reduced by the addition of the citric acid and conversion rate is improved. The inventive method has the characteristics of mild condition, strong specificity, few byproducts, high conversion rate and the like.