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Method for carrying out biologic decolor for electric mother liquor of sodium glutamate

A biological decolorization and mother liquor technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as high investment and operation costs, secondary pollution, and huge process equipment. Achieve the effect of reducing energy consumption, reducing costs, and eliminating sterilization operations

Inactive Publication Date: 2008-03-19
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the decolorization technologies of waste water containing Mylard pigment mainly include physical adsorption method, physical suspension method, oxidation method, electrochemical method, etc. These processes have huge equipment, relatively high investment and operating costs, and are likely to cause secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) After solid-liquid separation and clarification of the monosodium glutamate and other electric mother liquors, 1.0% (mass / mother liquor volume) powdered quicklime (CaO) is added to the monosodium glutamate and other electric mother liquors after the solids are separated, stirred and blown into air The stirring speed is 200 rpm, the stripping time is 1 hour, the air flow rate is 1L / L.min, the pH of the mother liquor is adjusted from the initial 1.8 to 4, and the supernatant is taken by centrifugation for later use.

[0026] (2) Inoculate the solid slanted versicolor Versicolor versicolor on the solid PDA medium in the petri dish for activation, let it stand for 5 days at 27°C, and use a puncher to punch holes on the petri dish covered with mycelium, and take 3 to 4 pieces of mycelia with a diameter of 0.5 to 1 cm are inserted into a conical flask (250 ml) containing 100 ml of liquid PDA medium, and cultured on a shaking table for 5 days at a temperature of 27°C and a ...

Embodiment 2

[0029] (1) Carry out mother liquor pretreatment according to the step (1) in embodiment 1, pH is adjusted to 4.5, and quicklime addition is 1.5%, and stripping time is 1.5 hours.

[0030] (2) According to the step (2) in Example 1, the liquid versicolor versicolor seeds were prepared.

[0031] (3) Dilute the mother liquor after pretreatment 10 times, COD is 15000mg / l, chromaticity (A 475nm ) is equal to 0.48, 250ml Erlenmeyer flask 40% liquid content, 8% inoculum, shaker culture 5 days, shaker rotating speed is 150 rev / mins, culture temperature is controlled at 27~28 ℃, after cultivating 5 days, decolorization rate is 53.5% %, the COD removal rate was 60.1%, and the dry weight of the bacteria was 6.2g / l.

Embodiment 3

[0033] (1) Carry out mother liquor pretreatment according to the step (1) in the embodiment 1, pH is adjusted to 5, and quicklime addition is 1.8%, and stripping time is 1 hour.

[0034] (2) According to the step (2) in Example 1, the liquid versicolor versicolor seeds were prepared.

[0035] (3) Dilute the mother liquor after pretreatment 10 times, COD is 15000mg / l, chromaticity (A 475nm ) is equal to 0.50, 250ml Erlenmeyer flask 40% liquid content, 10% inoculum, shaker culture for 5 days, shaker speed is 150 rev / min, culture temperature is controlled at 27~28 ℃, after cultivating for 5 days, the decolorization rate is 56.6% , the COD removal rate is 74.2%, and the dry weight of the bacteria is 6.8g / l.

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Abstract

This invention discloses a method for biological decolorization of equipotential mother solution of monosodium glutamate by Coriolus versicolor ecto-enzyme. The method comprises: (1) diluting the pretreated equipotential mother solution of monosodium glutamate; (2) adding 5-20 vol. % of Coriolus versicolor ecto-enzyme, and stirring at 27-28 deg.C, 130-220 rpm and 0.05-0.2 L / L.min air flow for 5-10 d to obtain decolorized equipotential mother solution of monosodium glutamate. Coriolus versicolor ecto-enzyme can synthesize substances required for its growth from the organic matters existing in the equipotential mother solution of monosodium glutamate, and can decolorize high-content pigments existing in the wastewater, thus realizing recovery and recycling of wastewater containing monosodium glutamate.

Description

technical field [0001] The invention belongs to the field of wastewater biological treatment, in particular to a method for biologically decolorizing the mother liquor of monosodium glutamate and the like by using extracellular enzymes produced by versicolor versicolor versicolor. Background technique [0002] Monosodium glutamate isoelectric mother liquor is the waste liquor after extracting glutamic acid from fermentation broth in the production process of monosodium glutamate. This mother liquor has the characteristics of "five highs, one low and deep color", that is, high COD, BOD 5 High, high sulfate content, high ammonia nitrogen content, high bacteria content, low pH characteristics. Since the starch substances in the fermentation raw materials react with protein to produce a large amount of pigment substances, the chroma of the mother liquor such as monosodium glutamate is very dark, and direct discharge will reduce the light transmittance of the water body and affec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 贾翠英丛威康瑞娟蔡昭玲
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI