Method for deciding hydrolyzation or not or hydrolyzation degree of soybean isoflavone glucoside
A technology for isoflavone glucoside and hydrolysis degree, which is applied in the field of judging whether soybean isoflavone glucoside is hydrolyzed or the degree of hydrolysis, can solve problems such as cumbersome process, and achieve the effect of avoiding cumbersome process
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Embodiment 1
[0019] (1) Take 1 # Aspegillus niger SFII (Sichuan Food Industry Institute) 3.357, 2 # Aspegillus niger SFII 3.360, 3 # Aspegillus niger SFII 3.313, 4 # Aspergillus niger SFII M227, Bacillus subtilis SFII 10210, and Rhizopus SFII 03125 were respectively in a medium containing 8% defatted soybean meal and natural pH at a temperature of 28 to 30 °C 200r / min, shake flask culture for 6 days.
[0020] (2) Using 70% ethanol (ethanol volume (mL): soybean meal weight (g)=6:1), ultrasonically extract the above-mentioned microbial fermentation culture medium at 40° C. for 40 minutes, respectively, and suction filtration to obtain ethanol The extract was concentrated under reduced pressure at 65°C until the concentrate did not contain ethanol to obtain an aqueous phase.
[0021] (3) Extract three times with the same volume of ethyl acetate as the water phase, combine the ethyl acetate phases and concentrate under reduced pressure to obtain a concentrated ethyl acetate phase.
[0022...
Embodiment 2
[0029] (1) Weigh 2.5 g of defatted soybean meal, extract with 70% ethanol (ethanol volume (mL): soybean meal weight (g) = 6:1), ultrasonically extract at 40° C. for 40 minutes, and suction filter to obtain an ethanol extract.
[0030] (2) Hydrolyze the above ethanol extract with 0.5, 1.0 and 2.0 moL / L of hydrochloric acid respectively in a water bath at 80°C to obtain 1 # Hydrolyzed sample, 2 # Hydrolyzed samples and 3 # Hydrolyzed samples.
[0031] (3) Adjust the pH of the hydrolyzed solution to 4-5 with NaOH, extract three times with the same volume of ethyl acetate as each hydrolyzed sample, combine the ethyl acetate phases and concentrate under reduced pressure to obtain a concentrated ethyl acetate phase.
[0032] (4) in GF 254 On a chromatographic plate, thin layer chromatography was performed on the concentrated ethyl acetate phase with a volume ratio of toluene: chloroform: acetone = 4: 1.8: 1.8 as a developing solvent. Observe the presence or absence or intensity ...
Embodiment 3
[0038] (1) Take 2.5g of defatted soybean meal, and process it with the method of Example 2 (1) to obtain an ethanol extract.
[0039] (2) Use 2.0moL / L hydrochloric acid to hydrolyze the above-mentioned ethanol extract in a water bath at 80°C for 0.5h, 1.0h, and 1.5h respectively to obtain 1 # Hydrolyzed sample, 2 # Hydrolyzed sample and 3 # Hydrolyzed sample.
[0040] (3) Process the hydrolyzed sample with the method of Example 2 (3) to obtain the concentrated phase of ethyl acetate.
[0041] (4) Obtain the following results as in Table 3 with the method of embodiment 2 (4).
[0042] Table 3 Contents of genistein and daidzein in each hydrolyzed sample after soybean meal was hydrolyzed by the same concentration of hydrochloric acid for different time
[0043]
[0044] Note: 1, 2, and 3 in the table represent the fluorescence of the third fluorescent spot from strong to weak
[0045] From the above results, it can be seen that when the soybean meal is hydrolyzed by the s...
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