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Application of katsumada galangal seed or its substitute extracts or its inclusive compound in pharmacy

An extract, the technology of cardamom, applied in the field of application of cardamom or its substitute extract or its contained compounds in pharmaceuticals

Inactive Publication Date: 2009-02-11
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] So far, there is no literature report to make any one of the above four compounds into medicine, only some preliminary researches on the theoretical aspects

Method used

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  • Application of katsumada galangal seed or its substitute extracts or its inclusive compound in pharmacy
  • Application of katsumada galangal seed or its substitute extracts or its inclusive compound in pharmacy
  • Application of katsumada galangal seed or its substitute extracts or its inclusive compound in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of Cardamom officinalis Extract and Separation and Purification of Four Monomer Compounds

[0047] Take 18kg of cardamom medicinal material, pulverize it, reflux extract with 70-90% industrial ethanol for three times, filter the extract while it is hot, combine the filtrates, recover the solvent under reduced pressure, and obtain 1300 g of dry extract (yield: 7.2%), which is cardamom Extract.

[0048] After the extract was diluted with water to form a suspension, it was extracted three times with petroleum ether, ether, ethyl acetate and n-butanol respectively to obtain petroleum ether, ether, ethyl acetate, n-butanol and aqueous solution. The fractions of petroleum ether, diethyl ether and ethyl acetate were subjected to silica gel column chromatography and eluted with petroleum ether-ethyl acetate gradient to obtain compounds CDK1, CDK2, CDK3 and CDK4.

Embodiment 2

[0049] Embodiment 2: drug to cytotoxicity test

[0050] 1. Experimental materials

[0051] 1) Compounds: CDK-1, CDK-2, CDK-3 and CDK-4 are monomeric compounds extracted and isolated from the medicinal material Cardamom, with a purity greater than 95%. Yellow or white powder. CDK-1, CDK-2, CDK-3 and CDK-4 were made into 50 mg / ml mother solution with DMSO, and diluted with culture medium to the required concentration before use. Positive control drug Ribavirin (RBV) is produced by Hubei Qianjiang Pharmaceutical Factory, white powder, made into 10mg / ml mother solution with double distilled water (DDW), positive control drug Acyclovir (ACV) is Hubei Qianjiang Pharmaceutical Factory production, white powder, mixed with double distilled water (DDW) to make 1mg / ml mother solution.

[0052] 2) Cells: MDCK cells; Vero cells, subcultured in our laboratory.

[0053] 3) Reagents, laboratory supplies and instruments:

[0054] Eagles MEM dry powder, glutamine are all products of GIBCO,...

Embodiment 3

[0068] Embodiment 3: The inhibitory test of medicine to influenza virus

[0069] 1) Use the same relevant experimental materials and cell culture methods as in Example 2 to carry out the experiment. Influenza virus type A / Jifang / 15 / 90(H3N2), A / Yuefang / 243 / 72(H3N2), A / Jingfang / 262 / 95(H1N1); Influenza virus type B / Jifang / 13 / 97.

[0070] 2) Experimental method:

[0071] Inoculate 96-well cell culture plate with 200,000-300,000 MDCK cells per milliliter, 0.1 ml per well, 37°C, 5% CO 2 Cultivate for 24 hours, discard the culture medium, add an appropriate amount of virus, after 2 hours of adsorption, discard the virus solution, add the influenza upper layer containing non-toxic concentrations of CDK-1, CDK-2, CDK-3 and CDK-4, and set up 5 dilutions in total degree, 3 wells per concentration, 37°C, 5% CO 2 to cultivate. When the lesions of the virus control group reach 4, observe the results, 4 is completely destroyed; 3 is 75%; 2 is 50%; 1 is 25%; 0 is no lesion. At the same...

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Abstract

The invention relates to a the novel use of katsumada galangal seed or its substitute extracts or its inclusive compound in pharmacy, more specifically the novel use of katsumadai seed extract or cardamonin, pinocembrin, 1,7-diphenyl-4,6-heptadiene-3-one and alpinetin compound in the preparation of medicament for resisting viral infection.

Description

technical field [0001] The present invention relates to the new use of the Chinese medicinal herb cardamom or its substitute extract or its contained compounds as the preparation of antiviral infection drugs, in particular to the Chinese medicinal herb cardamom extract or its contained cardamonin, chopinenin, 1,7-diphenyl The new application of base-4,6-heptadien-3-one and / or alpine compound as preparation of antiviral infection medicine. Background technique [0002] The Zingiberaceae Alpinia plant includes more than 20 kinds of plants such as cardamom grass, cardamom, yunnanensis and yunnanensis. [0003] Cardamom is the seed of Alpinia katsumadai, a plant belonging to the Zingiberaceae family. Used medicinally is the cardamom extract. There are several kinds of plants of the genus Alpinia that are usually substituted for cardamom in folk medicine, such as: Alpinia henryik.Schum.var.densihispida, Alpinia blepharocalyx and Alpiniablepharocalyx var.glabrior, etc. Similar ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9062A61P31/12A61P31/14A61P31/16A61P31/18A61P31/22A61K131/00
Inventor 王峥涛黄文哲李玉环
Owner CHINA PHARM UNIV
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