A method for separating and purifying alderone, pinenin, cardamonin and alpinol from cardamom

Abstract

The invention provides a method for separating and purifying alnustone, pinocembrin, cardamomin and alpinetin from alpinia katsumadai. Alpinia katsumadai is smashed, 70% ethanol is added for ultrasonic extraction, an extraction solution is filtered and subjected to vacuum concentration to obtain an alpinia katsumadai extract; the extract is dissolved with methanol, supercritical fluid chromatographic separation is conducted after filtering, a chromatographic column is a diol column, a mobile phase is supercritical CO2 fluid, and ethanol serves as a modifier. Carbon dioxide serves as a main mobile phase, a small quantity of organic solvent is added to serve as the modifier, harm to the environment and body health is small, recycling of carbon dioxide is easy, energy consumption is low, production cost is low, the production process is environmentally friendly, products are easy to recycle, and the obtained products are free of harmful matter and residues.

Description

technical field [0001] The invention belongs to the fields of chemical industry and pharmacy, and in particular relates to a supercritical fluid chromatography method for separating and purifying alderone, pinenin, cardamonin and alpinol from cardamom extract. Background technique [0002] Cardamom grass is the dry and nearly mature seed mass of cardamom grass, a plant in the ginger family. It is fragrant, pungent, and slightly bitter. It has the functions of drying dampness and invigorating the spleen, warming the stomach and relieving vomiting. Full of cold pain, belching and vomiting, not thinking about eating and other symptoms. Preliminary studies have shown that alpinol, joconin, cardamonin, and alderone have the highest content in cardamom, and have pharmacological effects such as anti-inflammatory, antibacterial, hypoglycemic, anti-oxidation, anti-radiation, anti-cancer, anti-tumor, and enhancing immunity . [0003] The separation methods of the effective component...

AI Technical Summary

Problems solved by technology

Although the purity of the compound obtained by column chromatography is high, the operation is cumbersome, the choice of solvent system in high-speed countercurrent chromatography is difficult, and the separation scale is difficult to expand

In addition, both methods need to consume a large amount of organic solvents. The recovery cost of organic solvents is high, recycling is difficult, and the residue of organic reagents in the product is serious, which is harmful to the environment and human health.

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