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Method for promoting Chinese wildrye callus differentiation rate and its special medium

A technology of differentiation medium and callus, applied in the directions of tissue culture, biochemical equipment and methods, horticultural methods, etc., can solve the problem of low tissue differentiation rate of Leymus chinensis, achieve simple and easy method, improve genetic transformation rate, The effect of increasing the differentiation rate

Inactive Publication Date: 2009-06-03
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for improving the callus differentiation rate of Leymus chinensis and its special medium, so as to solve the problem that the differentiation rate of Leymus chinensis is too low in the tissue culture process

Method used

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  • Method for promoting Chinese wildrye callus differentiation rate and its special medium

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Embodiment 1

[0015] Embodiment 1, tall chinensis callus culture differentiation and its effect

[0016] 1. Treatment of Leymus chinensis seeds

[0017] Select plump Leymus chinensis seeds, remove the seed coat, soak them in 75% alcohol for 45 seconds in an ultra-clean workbench, then rinse them with sterile water, and then sterilize them with 2% sodium hypochlorite for 10 minutes, shaking them constantly to ensure Disinfect thoroughly, then rinse with sterile water.

[0018] 2. Induction of callus

[0019] 600 sterile seeds were inoculated on N6 medium supplemented with 2.0 mg / L 2,4-D, 7.5 g / L agar, 30 g / L sucrose, pH 5.8, and cultured in constant temperature and dark at 25°C as the experimental group.

[0020] N6 medium: KNO 3 2800mg / L, (NH4) 2 SO 4 463mg / L, KH 2 PO 4 400mg / L, MgSO 4 ·7H 2 O185mg / L, CaCl 2 2H 2 O 165mg / L, Na 2 - EDTA 37.3mg / L, FeSO 4 ·7H 2 O 27.8mg / L, MnSO 4 ·H 2 O 4.4mg / L, ZnSO 4 ·7H 2 O 1.5mg / L, H 3 BO 3 1.6mg / L, KI 0.8mg / L, vitamin B1 1mg / L, vit...

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Abstract

The invention discloses a method for increasing the differentiation rate of beard grass callus and the special-purpose culture medium. The said culture medium is the N6 solid culture medium which comprises 0.3-2.5 mg / l 6-BA and 0.3-2.5 mg / l KT, and replaces cane sugar with 30-90 g / l malt sugar. The said method comprises: placing the callus into pre-differentiation culture medium for 20-30 days; then inoculating into the said culture medium for differentiation and culturing for germination. The said pre-differentiation culture medium is the 2, 4-D basic culture medium with addictive 0.05-1 mg / l. The invention is characterized by the simple process and operation, and effective increase for differentiation rate.

Description

technical field [0001] The invention relates to a method for increasing the callus differentiation rate of Leymus chinensis and a special culture medium thereof. Background technique [0002] Leymus chinensis belongs to monocotyledonous plants, Gramineae, Gramineae, Bluegrass family, Barley family, Wildgrass subfamily, Lyymus genus, and is one of the important grassland constructing species in the eastern steppe region of Eurasia. As an important pasture resource, Leymus chinensis has many advantages such as high yield, strong stress resistance, good animal palatability, and rich nutrition; at the same time, Leymus chinensis also has strong adaptability to different ecological environments, which is very important for improving Grassland ecological environment and control of salinized land are of great significance. [0003] Over the years, people have mainly domesticated and improved Leymus chinensis populations and bred fine varieties through traditional breeding techniqu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 刘公社李晓峰苏蔓齐冬梅
Owner INST OF BOTANY CHINESE ACAD OF SCI
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