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Process for breaking cell or breaking wall and its mechanical apparatus

A cell and raw material technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve problems such as breaking walls and cutting cells, and achieve high efficiency and good working results

Inactive Publication Date: 2009-06-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] When it comes to knife cutting, although the slices made by biological microscopes in the past are organ and tissue slices, objectively, they will also have the effect of knife cutting to break cells and walls

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Example 1. Processing of broken ultra-thin slices of ginseng and American ginseng. The fresh or rehydrated ginseng is properly frozen or not, and it is clamped by the material fixing device of the cutting and shaving mechanical device, and cut and shaved by the knife to obtain all the broken ultra-thin ginseng slices. First, the initial observation of the microscopic section of the microscope shows that the average cell size of American ginseng is 50 microns, the cutting distance (cutting distance) is 46-66 microns, and the cell wall breaking rate is 100-83%.

Embodiment 2

[0034] Example 2. Pollen. According to the preliminary observation of the microscopic section under the microscope, the pine pollen is 80-100 microns in size, with an average of 88 microns.

[0035] Freezing liquid formula, sodium chloride 0.1%, pectin 1%, glycerol 1%, lecithin emulsified peanut oil 1%, residual water.

[0036] The freezing mold made of 2mm thick stainless steel iron sheet has a 1x1 cm cross-section and a long strip of 18 cm long. The fish eggs are vibrated and pressed, immersed or injected into the above freezing liquid, and frozen below -20℃, not less than 6 hours, 29 Slightly soak in warm water for demoulding, freeze intensively and prepare for cutting.

[0037] The frozen material strip is clamped and fixed on the material fixing device of the cutting and shaving mechanical device, the knife distance (knife distance) is 76-116 microns, and the crushing rate is 100-76%.

Embodiment 3

[0038] Example 3 is the same as Example 2, except that the freezing liquid formulation is 100% water.

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Abstract

The invention is a cell and cell wall breaking method and its mechanical device, a method of breaking cells and cell walls by cutting, using sharp blade at a very fine cutter space, less than, equal to slightly greater than the size of the cells to be broken to cut all or most of them one in two or two in three, and achieving the effect of breaking cells and cell walls. It primely makes each part of the substance in the broken cell maintain original property in situ, thus beneficial to fully and normally use nutrient constituents and substances in the cell.

Description

Technical field [0001] A cell rupture and wall breaking method belongs to the fields of food and pharmaceutical raw material processing and processing machinery manufacturing. A23L1 / 076 Background technique [0002] It is organisms that provide humans with most of the value of survival resources. Biology is composed of cells. Cells are composed of cell membranes, nuclei, and cytoplasm. Plant cells generally have cell walls that are difficult to break. [0003] When humans want to ingest and use the substances inside these cells, the integrity of the cell structure will have a hindering effect. For example, the toughness of the pollen wall makes it difficult for the essence of the inside to be effectively used, so people have studied a variety of Various methods of breaking walls. Just give a few examples of Chinese patents: Publication No. 1377654, which has a process flow of two steps: pollen solution sterilization and enzymatic hydrolysis; Dry the Ganoderma lucidum spore powder...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A23L1/317A23P1/02G01N1/06
Inventor 辛益群
Owner SHANDONG UNIV