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Method for inductrialized separation of purified lactoferrins from cattle colostrum

A lactoferrin, separation and purification technology, applied in protein food processing, animal protein processing, protein food ingredients and other directions, can solve the problems of hindering the popularization and application of lactoferrin, difficult to achieve industrialization, small processing capacity, etc., to improve health awareness , Reduce the effect of co-precipitation and strong dirt holding capacity

Inactive Publication Date: 2009-11-11
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] It can be seen that although some separation techniques can produce high-purity lactoferrin on a laboratory scale, the processing volume is small, the cost is high, and it is difficult to realize industrialization; while the ultrafiltration method has a large output but low purity, which hinders the production of lactoferrin. Promotion and application of protein

Method used

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  • Method for inductrialized separation of purified lactoferrins from cattle colostrum

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Isolate and purify lactoferrin according to the above-mentioned process method in the instruction manual. Skimmed colostrum volume: 100L, lactoferrin content 1.50g / L, enzymatically remove casein to obtain 90L of enzymatic whey, two-step membrane separation to obtain concentrated solution 18L, lactoferrin concentration 6.91g / L, lactoferrin The protein recovery rate (based on the content of lactoferrin in colostrum) is 82.92%, and the purity of lactoferrin in the concentrate relative to the total protein in the concentrate is 31.40%. After chromatographic separation, desalination, concentration and freeze-drying of the concentrated solution, 113.18 g of refined lactoferrin was obtained with a purity of 94.20%. The recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) was 71.08%.

Embodiment 2

[0022] The operation is the same as in Example 1, the amount of defatted colostrum: 300L, the content of lactoferrin is 1.48g / L, the enzymatic whey is obtained after removing casein by enzymatic method, 272L of enzymatic whey is obtained, and the concentrated solution is obtained after two-step membrane separation. 53L, the concentration of lactoferrin is 7.05 g / L, the recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) is 84.16%, and the purity of lactoferrin in the concentrate relative to the total protein in the concentrate is 32.91%. After chromatographic separation, desalination, concentration and freeze-drying of the concentrated solution, 350.35 g of refined lactoferrin was obtained with a purity of 95.92%. The recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) was 75.72%.

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Abstract

The invention discloses a method for industrial separation and purification of lactoferrin from bovine colostrum, which belongs to the technical field of industrial separation and purification of lactoferrin in bovine colostrum. The invention uses bovine colostrum defatted whey as raw material, adopts enzymatic method to remove casein, combines membrane separation technology and industrial chromatographic technology, industrially separates lactoferrin with a purity of more than 90% from bovine colostrum, and involves membrane separation The application of modern biological integration technologies such as technology and industrial chromatographic technology in the deep processing of agricultural products, the method of the present invention is suitable for industrial scale application, and its advantages are fast and effective, moderate cost, high purity and high recovery rate. The invention has important significance for promoting deep processing of dairy products and comprehensive utilization of colostrum resources, improving the health level of Chinese people, especially infants, and researching and developing functional food ingredients.

Description

technical field [0001] A method for industrial separation and purification of lactoferrin from bovine colostrum, which involves the application of modern biological integration technologies such as membrane separation technology and industrial chromatography technology in the deep processing of agricultural products, and belongs to the technical field of industrial separation and purification of lactoferrin in bovine colostrum . Background technique [0002] The dairy industry generally believes that the milk produced within 7 days after giving birth is called bovine colostrum. Colostrum is a very specific type of milk whose chemical composition and processing characteristics are very different from ordinary milk. The color of colostrum is yellow and thick, even with a small amount of blood red, and has a special milky smell. The milk solid content of colostrum is higher than that of normal milk, and the content of globulin, albumin and inorganic salts is particularly high...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/08A23J3/34
Inventor 卢蓉蓉许时婴方民杨瑞金
Owner JIANGNAN UNIV