Antimicrobial compositions
A technology of mixtures and substances, applied in the direction of resistance to vector-borne diseases, active ingredients of aldehydes, organic active ingredients, etc., can solve the problems of no biocidal effect, expensive, etc.
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Embodiment 1
[0099] Test as described below Figure 1-3 Each anionic shampoo sample in . Follow the steps below to prepare a standardized mixed bacterial solution. Three agar stabs of Staphylococcus aureus (ATCC #6538), Pseudomonas aeruginosa (ATCC #9027) and Escherichia coli (ATCC #8739) were each incubated at about 35°C for about 24 hours. Each puncture was then washed with 3 mL of 0.85% sterile saline solution. Pool the washes from the three punctures together to form the biota mixture. The absorbance of the organism mixture at 530 nm was adjusted to about 1.00 by adding saline. Calibrate the spectrometer with a saline blank. A standardized mixed bacterial solution was prepared by mixing together 5 mL aliquots of this organism mixture. Then, 0.2 mL of the standardized mixed bacterial solution was inoculated and mixed in 40 g samples of each shampoo. One gram of this mixture was added to a sterile 20 x 150 mm screw cap test tube.
[0100] Add 9 mL of sterile D / E neutralizer broth ...
Embodiment 2
[0105] Test as described below Figure 4-7 Each anionic shampoo sample in . Follow the steps below to prepare a standardized mixed bacterial solution. Two Candida albicans agar stabs and four Aspergillus niger agar slants were incubated at about 25°C for about 48 hours and 7 days, respectively. Each puncture was then rinsed with 3 mL of 0.85% sterile saline solution, collected and macerated in a tissue grinder. A sufficient amount of 0.85% saline solution was added to each puncture and each inoculum of Candida albicans and Aspergillus niger was visually counted under a microscope with a Neubauer Hemocytometer. Equal volumes of each standardized inoculum of C. albicans and A. niger were mixed together to form a standardized mixed fungal solution.
[0106] A 40 gram sample of each shampoo was inoculated with 0.4 mL of the standardized mixed fungal solution and mixed. One gram of this mixture was added to a sterile 20 x 150 mm screw cap test tube.
[0107] Add 9 mL of steril...
Embodiment 3
[0111] The procedure described in Example 1 was repeated with the preservative formulations listed in Table 1 below. Adjust the shampoo pH to 6.5. The results are also listed in Table 1.
[0112] Table 1
[0113] Preservatives
0 days
cfu / g
7 days
cfu / g
14 days
cfu / g
28 days
cfu / g
0.3 wt% blend with 75% sorbus
Potassium Erythorbate and 25% Sodium Erythorbate
1-3×10 6
＜10
＜10
＜10
0.3% by weight mixture containing 75% Benzene
sodium erythorbate and 25% sodium erythorbate
1-3×10 6
＜10
＜10
＜10
0.45% by weight sodium erythorbate
1-3×10 6
＞3×10 6
＞3×10 6
＞3×10 6
0.45% by weight sodium benzoate
1-3×10 6
1×10 5
7×10 5
＜10
0.45% by weight potassium sorbate
1-3×10 6
1×10 5
6×10 4
not detected
Unpreserved Shampoo
1-3×10 6
...
PUM
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