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Induction of adventitious bud and body cell embryo of north American red fir leave and proces sof tree regenerating

An embryo induction and somatic cell technology, applied in the fields of plant regeneration, botanical equipment and methods, plant cells, etc., can solve the problems of unsuccessful embryo induction of adventitious buds and somatic cells, difficult to meet production applications, and slow reproduction speed.

Inactive Publication Date: 2007-07-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, traditional propagation methods such as seeds or cuttings are used in production, but the propagation speed is slow and it is difficult to meet the needs of production applications
[0003] Although the induction of somatic embryos from North American Sequoia has been reported, the materials are limited to immature embryos and zygotic embryos, and only Induction of callus, adventitious buds and somatic embryos has not been successful

Method used

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  • Induction of adventitious bud and body cell embryo of north American red fir leave and proces sof tree regenerating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Inoculate the shoot tip of Sequoia 1-2cm on MS medium without phytohormone, and carry out subculture in a culture room with light intensity of 1500-2000lx, photoperiod of 18h / d, and temperature of 25±2℃ Cultivate; take the test-tube plantlets subcultured on MS medium for 28 days, cut the needles at the top into small pieces, and contact the medium with the abaxial surface to induce adventitious buds; the basic medium is SH; the additional plant hormone is: BA0 .46mg / L, KT0.18mg / L and IBA0.2mg / L. Take the test-tube plantlets subcultured on MS medium for 32 days, cut the top needles into small pieces, and contact the medium with the abaxial surface to induce somatic embryos; the basic medium is SH; the additional plant hormone is: BA0. 5mg / L and IBA0.45mg / L. After inoculation, culture in dark for 30 days; then transfer to fresh culture medium, and then culture in dark for 20 days, then transfer to light culture. Investigate the number of adventitious buds and somatic em...

Embodiment 2

[0019] Inoculate the shoot tip of Sequoia 1-2cm on MS medium without phytohormone, and carry out subculture in a culture room with light intensity of 1500-2000lx, photoperiod of 18h / d, and temperature of 25±2℃ to cultivate;. Take the test-tube plantlets subcultured on MS medium for 29 days, cut the top needles into small pieces, and contact the medium with the abaxial surface to induce adventitious buds; the basic medium is SH; the additional plant hormone is: BA0.5mg / L, KT0.2mg / L and IBA0.18mg / L. Take the test-tube plantlets subcultured on MS medium for 35 days, cut the apical needles into small pieces, and contact the medium with the abaxial surface to induce somatic embryos; the basic medium is SH; the additional plant hormone is: BA0. 45mg / L and IBA0.5mg / L. After inoculation, culture in dark for 30 days; then transfer to fresh culture medium, and then culture in dark for 20 days, then transfer to light culture. Investigate the number of adventitious buds and somatic em...

Embodiment 3

[0021] Inoculate the shoot tip of Sequoia 1-2cm on MS medium without phytohormone, and carry out subculture in a culture room with light intensity of 1500-2000lx, photoperiod of 18h / d, and temperature of 25±2℃ to cultivate;. Take the test-tube plantlets subcultured on MS medium for 30 days, cut the top needles into small pieces, and contact the medium with the abaxial surface to induce adventitious buds; the basic medium is SH; the additional plant hormone is: BA0.5mg / L, KT0.2mg / L and IBA0.2mg / L. Take the test-tube plantlets subcultured on MS medium for 38 days, cut the top needles into small pieces, and contact the medium with the abaxial surface to induce somatic embryos; the basic medium is SH; the additional plant hormone is: BA0. 5mg / L and IBA0.5mg / L. After inoculation, culture in dark for 30 days; then transfer to fresh culture medium, and then culture in dark for 20 days, then transfer to light culture. Investigate the number of adventitious buds and somatic embryos...

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Abstract

The present invention is process of inducing indefinite bud and somatic embryo of North America redwood leaf and regenerating plant, and belongs to the field of forestry cell engineering breeding technology. The technological scheme includes inoculating stem apex of North America redwood in MS culture medium for secondary culture, taking 28-32 day and 30-40 day cultured test tube plantlet and making the far axial plane contact with the culture medium to dark culture for 30 days, transferring to fresh culture medium to culture for other 20 days, and transferring for light culture. In SH culture medium containing BA 0.5 mg / L+KT 0.2 mg / L+IBA 0.2 mg / L and BA 0.5 mg / L+IBA 0.5 mg / L, indefinite bud and somatic embryo are induced successfully. The present invention makes it possible to realize the large scale, short period and low cost industrial production of North America redwood nursery stock, and has important cytology significance.

Description

technical field [0001] The invention relates to a method for inducing adventitious buds and somatic embryos from North American redwoods, belonging to the field of forestry cell engineering breeding. Background technique [0002] North American Sequoia (Sequoia sempervirens Endl.), also known as Coastal Sequoia, Evergreen Sequoia, California Sequoia, commonly known as Sequoia, is the tallest tree species in the world and is known as "the world of red wood"; its trunk is straight and round, The branches are small and the growth speed is fast. It is a world-renowned timber tree species, and the tree species has a beautiful tree shape, and it is also an excellent tree species for garden greening. Since 1840, many countries have introduced and achieved success. At present, traditional propagation methods such as seeds or cuttings are used in production, but the propagation speed is slow and it is difficult to meet the needs of production applications. [0003] Although the ind...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01H4/00
Inventor 尹伟伦夏新莉刘翠琼
Owner BEIJING FORESTRY UNIVERSITY
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