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Vintage yeast CWY132 and application of the same in preparation of 2-phenethylol by microorganism fermentation

A technology of Saccharomyces cerevisiae and phenylethanol, which is applied in the direction of fermentation and fungi, which can solve problems affecting product quality, human health and environmental hazards, and achieve the effects of low price, low production investment and short fermentation cycle

Inactive Publication Date: 2007-08-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the end of the last century, consumers have higher and higher requirements for "natural" products. In the international market, the demand for natural 2-phenylethanol is very considerable, and the price is as high as 1000$ / kg; due to the long-term dominance of domestic chemical products , so chemically synthesized 2-phenylethanol still accounts for the vast majority, but chemically synthesized 2-phenylethanol uses carcinogens such as benzene and styrene as raw materials, which is harmful to human health and the environment. In addition, chemically synthesized 2-phenylethanol Phenylethyl alcohol often contains some by-products that are difficult to remove, which seriously affects product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Utilize Saccharomyces cerevisiae CWY132 to ferment and produce natural 2-phenylethanol, the steps are as follows:

[0037] 1. Slant culture: Inoculate Saccharomyces cerevisiae CWY132 on the solid slant of YPAD, culture at 28°C for 60 hours, and store in a 4°C refrigerator;

[0038] 2. Solid plate culture: Saccharomyces cerevisiae CWY132 preserved on a slant was inoculated on a YPAD solid plate with an inoculation ring, and cultured at 28°C for 48 hours to form a mature single colony, which is the solid plate strain;

[0039] 3. Liquid culture: Pick a single colony of Saccharomyces cerevisiae CWY132 on a solid plate and inoculate it into a conical flask containing 100 ml of YPAD liquid medium with an inoculation loop, and culture it with stirring at 28°C for 16 hours to become a liquid Strains;

[0040] 4. First-level liquid seed culture: the liquid bacteria were inserted into a triangular flask equipped with 500 milliliters of YPAD liquid medium according to an 8% inoc...

Embodiment 2

[0045] Utilize Saccharomyces cerevisiae CWY132 to ferment and produce natural 2-phenylethanol, the steps are as follows:

[0046] 1. Slant culture: Inoculate Saccharomyces cerevisiae CWY132 on the solid slope of YEPD, culture at 30°C for 48 hours, and store in a 4°C refrigerator;

[0047] 2. Solid plate culture: Saccharomyces cerevisiae CWY132 preserved on a slant was inoculated on a YEPD solid plate with an inoculation loop and cultured at 30°C for 36 hours to form a mature single colony, which is the solid plate strain;

[0048] 3. Liquid culture: Pick a single colony of Saccharomyces cerevisiae CWY132 on a solid plate and inoculate it into a conical flask containing 100 ml of YEPD liquid medium with an inoculation loop, and culture it with stirring at 30°C for 14 hours to obtain a liquid Strains;

[0049] 4. First-level liquid seed culture: the inoculum amount of the liquid bacteria is inserted into a conical flask equipped with 500 milliliters of YEPD liquid medium accord...

Embodiment 3

[0054] Utilize Saccharomyces cerevisiae CWY132 to ferment and produce natural 2-phenylethanol, the steps are as follows:

[0055] 1. Slant culture: Inoculate Saccharomyces cerevisiae CWY132 on the solid slope of YPAD, culture at 32°C for 40 hours, and store in a 4°C refrigerator;

[0056] 2. Solid plate culture: Saccharomyces cerevisiae CWY132 preserved on a slant was inoculated on a YPAD solid plate with an inoculation ring, and cultured at 32°C for 30 hours to form a mature single colony, which is the solid plate strain;

[0057] 3. Liquid culture: Pick a single colony of Saccharomyces cerevisiae CWY132 on a solid plate and inoculate it into a conical flask containing 100 ml of YPAD liquid medium with an inoculation loop, and culture it with stirring at 32°C for 16 hours to become a liquid Strains;

[0058] 4. First-level liquid seed culture: the inoculum amount of the liquid bacteria was inserted into a conical flask equipped with 500 milliliters of YEPD liquid medium acco...

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PUM

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Abstract

The invention discloses a saccharomyces cerevisiae mutagenesis strain--saccharomyces cerevisiae CWY132 and application to prepare 2-benzyl carbinol, which is characterized by the following: the CWY132 (Saccharomyces cerevisiae CWY132) is kept in Chinese typical culture preservation center; the preservation date is 12.15 2006; the keeping number is CCTCC NO: M206136; the ferment circle is short; the ferment temperature range is wider. This invention does not possess special demand to device, which can be used to general ferment plant.

Description

(1) Technical field [0001] The invention relates to a saccharomyces cerevisiae mutagenic strain—saccharomyces cerevisiae CWY132, and its application in microbial fermentation to prepare 2-phenylethanol. (2) Background technology [0002] 2-Phenylethyl alcohol is a kind of aromatic alcohol with rose fragrance. It is widely used in rose fragrance and other types of fragrance formulations in daily chemical and food industries. It is also an indispensable ingredient in all floral fragrance blends and high-end perfumes. Since the end of the last century, consumers have higher and higher requirements for "natural" products. In the international market, the demand for natural 2-phenylethanol is very considerable, and the price is as high as 1000$ / kg; due to the long-term dominance of domestic chemical products , so chemically synthesized 2-phenylethanol still accounts for the vast majority, but chemically synthesized 2-phenylethanol uses carcinogens such as benzene and styrene as r...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/22
Inventor 崔志峰杨霄汪琨朱廷恒
Owner ZHEJIANG UNIV OF TECH
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