Method for extracting genipin and geniposide from gardenia jasminoides

A technology of geniposide and genipin, which is applied in the field of preparation of converting geniposide into genipin, can solve the problems of environmental pollution, cost increase, low purity, etc., and achieves the avoidance of environmental pollution, convenient operation, The effect of simple process

Inactive Publication Date: 2007-09-05
GUILIN NATURAL INGREDIENTS CORP
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of active ingredients in natural products by this method has certain limitations. The yield of the compounds obtained by the separation is low, the purity is not high, the active ingredients are

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting genipin and geniposide from gardenia jasminoides
  • Method for extracting genipin and geniposide from gardenia jasminoides

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

Example 1

1) place 10 times of the pure water in the pure water of its weight after the Chinese medicine gardenia is smashed and extract 2 hours, temperature is controlled at 80 ℃, obtains extracting solution;

2) The extract is filtered and concentrated;

3) with the concentrated filtrate, by column chromatography, the macroporous resin of DA201 type is adsorbed with the model;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the pasty geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1 / 20 of the weight of the raw material, the concentration of the enzyme is 1mg / ml, and the enzymolysis temperature is 55°C;

7) the solution after enzymolysis is adsorbed by column chromatography w...

Example Embodiment

Example 2

1) place 8 times of the 30% methanol of its weight after the Chinese medicine gardenia smashed and extract 1 hour under reflux, and temperature is controlled at 60 DEG C to obtain an extract;

2) The extract is filtered and concentrated;

3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorbent resin of type H107;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the paste-like geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1 / 20 of the weight of the raw material, the concentration of the enzyme is 1mg / ml, and the enzymolysis temperature is 60°C;

7) Adsorb the solution after enzymolysis with H107 resin through co...

Example Embodiment

Example 3

1) The fresh gardenia fruit is placed in 10 times of 50% ethanol of its weight and heated to reflux for extraction for 2 hours, and the temperature is controlled at 70°C to obtain an extract;

2) The extract is filtered and concentrated;

3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorption resin of HP20SS;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the pasty geniposide with water, add β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1 / 10 of the weight of the raw material, the concentration of the enzyme is 1mg / ml, and the enzymolysis temperature is 50 ° C;

7) The solution after enzymolysis is adsorbed by column chromatography with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A process for preparing geniposide and genipin from capejasmine fruit includes such steps as extracting, concentrating, chromatography, and freeze-drying to obtain geniposide, or extracting, concentrating, enzymolyzing, chromatography, and freeze- drying to obtain genipin.

Description

technical field The invention relates to a preparation method for extracting geniposide and genipin from gardenia, specifically, to a method for obtaining geniposide from gardenia, and further, converting geniposide into jing The preparation method of Nepin. Background technique Gardenia is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a plant of Rubiaceae (Rubiaceae), oblong or oval, dark red or yellow-red in color. Gardenia belongs to the first batch of food and medicine dual-use resources promulgated by the Ministry of Health, and is a traditional Chinese medicine. "(Compendium of Materia Medica)" has detailed records: Gardenia has the functions of promoting gallbladder and protecting liver, clearing heat and detoxifying, lowering blood pressure, antibacterial and anti-inflammatory. Traditional Chinese medicine is clinically used to treat jaundice hepatitis, sprains, high blood pressure, diabetes, etc. There are many chemical components in Gard...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07H17/04C07H1/08C12P17/06
Inventor 孙步祥汤凌志陈振鹏吴柏峰易永基王智慧
Owner GUILIN NATURAL INGREDIENTS CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap