Method for extracting genipin and geniposide from gardenia jasminoides

A technology of geniposide and genipin, which is applied in the field of preparation of converting geniposide into genipin, can solve the problems of environmental pollution, cost increase, low purity, etc., and achieves the avoidance of environmental pollution, convenient operation, The effect of simple process

Inactive Publication Date: 2007-09-05
GUILIN NATURAL INGREDIENTS CORP
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AI-Extracted Technical Summary

Problems solved by technology

The extraction of active ingredients in natural products by this method has certain limitations. The yield of the compounds obtained by the separation is low, the purity is not high, the active ingredients are ...
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Abstract

A process for preparing geniposide and genipin from capejasmine fruit includes such steps as extracting, concentrating, chromatography, and freeze-drying to obtain geniposide, or extracting, concentrating, enzymolyzing, chromatography, and freeze- drying to obtain genipin.

Application Domain

Technology Topic

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  • Method for extracting genipin and geniposide from gardenia jasminoides
  • Method for extracting genipin and geniposide from gardenia jasminoides

Examples

  • Experimental program(5)

Example Embodiment

Example 1
1) place 10 times of the pure water in the pure water of its weight after the Chinese medicine gardenia is smashed and extract 2 hours, temperature is controlled at 80 ℃, obtains extracting solution;
2) The extract is filtered and concentrated;
3) with the concentrated filtrate, by column chromatography, the macroporous resin of DA201 type is adsorbed with the model;
4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;
5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;
6) after diluting the pasty geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/20 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 55°C;
7) the solution after enzymolysis is adsorbed by column chromatography with the macroporous resin of type DA201;
8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;
9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.

Example Embodiment

Example 2
1) place 8 times of the 30% methanol of its weight after the Chinese medicine gardenia smashed and extract 1 hour under reflux, and temperature is controlled at 60 DEG C to obtain an extract;
2) The extract is filtered and concentrated;
3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorbent resin of type H107;
4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;
5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;
6) after diluting the paste-like geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/20 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 60°C;
7) Adsorb the solution after enzymolysis with H107 resin through column chromatography;
8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;
9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.

Example Embodiment

Example 3
1) The fresh gardenia fruit is placed in 10 times of 50% ethanol of its weight and heated to reflux for extraction for 2 hours, and the temperature is controlled at 70°C to obtain an extract;
2) The extract is filtered and concentrated;
3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorption resin of HP20SS;
4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;
5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;
6) after diluting the pasty geniposide with water, add β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/10 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 50 ° C;
7) The solution after enzymolysis is adsorbed by column chromatography with HP20SS resin;
8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;
9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.
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