Method for extracting genipin and geniposide from gardenia jasminoides

A technology of geniposide and genipin, which is applied in the field of preparation of converting geniposide into genipin, can solve the problems of environmental pollution, cost increase, low purity, etc., and achieves the avoidance of environmental pollution, convenient operation, The effect of simple process

Inactive Publication Date: 2007-09-05

GUILIN NATURAL INGREDIENTS CORP

0 Cites 18 Cited by

AI-Extracted Technical Summary

Problems solved by technology

The extraction of active ingredients in natural products by this method has certain limitations. The yield of the compounds obtained by the separation is low, the purity is not high, the active ingredients are ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Abstract

A process for preparing geniposide and genipin from capejasmine fruit includes such steps as extracting, concentrating, chromatography, and freeze-drying to obtain geniposide, or extracting, concentrating, enzymolyzing, chromatography, and freeze- drying to obtain genipin.

Application Domain

Sugar derivativesFermentation +1

Technology Topic

ChemistryFreeze dry +5

Image

Examples

Experimental program(5)

Example Embodiment

Example 1

1) place 10 times of the pure water in the pure water of its weight after the Chinese medicine gardenia is smashed and extract 2 hours, temperature is controlled at 80 ℃, obtains extracting solution;

2) The extract is filtered and concentrated;

3) with the concentrated filtrate, by column chromatography, the macroporous resin of DA201 type is adsorbed with the model;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the pasty geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/20 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 55°C;

7) the solution after enzymolysis is adsorbed by column chromatography with the macroporous resin of type DA201;

8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;

9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.

Example Embodiment

Example 2

1) place 8 times of the 30% methanol of its weight after the Chinese medicine gardenia smashed and extract 1 hour under reflux, and temperature is controlled at 60 DEG C to obtain an extract;

2) The extract is filtered and concentrated;

3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorbent resin of type H107;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the paste-like geniposide with water, adding β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/20 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 60°C;

7) Adsorb the solution after enzymolysis with H107 resin through column chromatography;

8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;

9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.

Example Embodiment

Example 3

1) The fresh gardenia fruit is placed in 10 times of 50% ethanol of its weight and heated to reflux for extraction for 2 hours, and the temperature is controlled at 70°C to obtain an extract;

2) The extract is filtered and concentrated;

3) the concentrated filtrate is adsorbed by column chromatography with a small particle size synthetic adsorption resin of HP20SS;

4) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with 15-20% ethanol solution to collect the eluate;

5) Concentrating the eluent to obtain paste-like geniposide, then obtain the geniposide of columnar crystals by freeze-drying;

6) after diluting the pasty geniposide with water, add β-glucosidase to carry out enzymolysis, the consumption of the enzyme is 1/10 of the weight of the raw material, the concentration of the enzyme is 1mg/ml, and the enzymolysis temperature is 50 ° C;

7) The solution after enzymolysis is adsorbed by column chromatography with HP20SS resin;

8) Wash the resin with deionized water until the effluent liquid is colorless, and then eluate with an ethanol solution with a concentration of 10-20% to collect the eluate;

9) Concentrate the eluate, and then freeze-dry to obtain powdered genipin.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

Classification and recommendation of technical efficacy words

High purity
High yield

Method for extracting genipin and geniposide from gardenia jasminoides

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Method for purifying sea-mussel mucin by carboxymethyl ion exchange chromatography

Composite molecular sieve in ten-element cyclic structure and its synthesis process

Disproportionation/transalkylation of aromatic hydrocarbons

Method and device for processing slaughter products

Catalyzer for preparing olefine in low carbon number through catalytic cracking and its preparing method and application

Classification and recommendation of technical efficacy words

Methods of Preparing Renewable Butadiene and Renewable Isoprene

Control of impurities in reaction product of rhodium-catalyzed methanol carbonylation

Preparation of maytansinoid antibody conjugates by a one-step process

Glucosyl Stevia Composition

Monoamino compound and organic luminescence device using the same

Coreless cavity substrates for chip packaging and their fabrication

Nanoparticle synthesis and the formation of inks therefrom

Three-dimensional-mapping two-dimensional-scanning lidar based on one-dimensional-steering optical phased arrays and method of using same

Lock and Key Through-Via Method for Wafer Level 3 D Integration and Structures Produced

Direct and selective production of ethyl acetate from acetic acid utilizing a bimetal supported catalyst