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Reagent kit for quickly testing protein content of milk powder and method for making same

A protein content and kit technology, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the harm of inferior milk powder, lack of detection methods, etc., to achieve the effect of low detection cost, simple equipment, and short detection time.

Inactive Publication Date: 2007-09-12
BEIJING ZHIYUNDA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in some small towns and villages, due to the lack of simple and effective detection methods, some low-quality milk powder has endangered the health of people, especially infants, and some have caused serious consequences

Method used

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  • Reagent kit for quickly testing protein content of milk powder and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Preparation comprises the test kit of 6 color band color comparison cards

[0044] There are 6 color bands in the color comparison card in the milk powder protein content rapid detection kit, which respectively represent the protein concentration: 0, 1.5, 3.0, 4.5, 6.0 and 7.5 mg / ml; the sampling volume of the milk powder sample is 30mg, the final solution volume is 4ml; then the protein content of the milk powder samples corresponding to the color chart is 0, 5, 10, 15, 20 and 25wt%.

[0045] Production of standard color charts:

[0046] First prepare 10% sodium hydroxide solution for later use.

[0047] Second, prepare the biuret reagent. Weigh 1.5g copper sulfate (CuSO 4 ·5H 2 O, analytically pure, purchased from Beijing Chemical Plant) and 6.0g sodium potassium tartrate (NaKC 4 h 4 o 6 4H 2 O, analytically pure, purchased from Beijing Chemical Reagent Company), dissolved in 500ml of water, then added 300ml of 10% sodium hydroxide solution under...

Embodiment 2

[0054] Embodiment 2: prepare the kit of 3 color bands color comparison card

[0055] Set the colorimetric card in the milk powder protein content rapid detection kit to have 3 color bands, respectively representing the protein concentration: 4.0, 6.0 and 8.0 mg / ml; the sampling volume of the milk powder sample is 80 mg, and the final color developing solution The volume is 10ml; then the protein content of the milk powder samples corresponding to the color chart is 10, 15 and 20wt%.

[0056] Production of standard color charts:

[0057] Prepare the biuret reagent according to the method in Example 1 for subsequent use.

[0058] Then, accurately weigh a certain amount of milk powder with a known protein content of 24.38 wt%, and prepare a 4.0 mg / ml milk powder standard protein solution. Then a certain amount of bovine serum albumin (purchased from Roche Company) was weighed to prepare standard protein solutions with a total protein content of 6.0 and 8.0 mg / ml sequentially wi...

Embodiment 3

[0063] Embodiment 3: prepare the kit of 4 color bands color comparison card

[0064] Set the color comparison card in the milk powder protein content rapid detection kit to have 4 color bands, respectively representing the protein concentration: 4.0, 6.0, 8.0 and 10.0mg / ml; the sampling amount of the milk powder sample is 160mg, and the final display The volume of the color solution is 25ml; then the protein content of the milk powder samples corresponding to the color card is 10, 15, 20 and 25 wt%.

[0065] Production of standard color charts:

[0066] According to the method in Example 1, biuret color developing solution was prepared for future use.

[0067] Then, accurately weigh a certain amount of milk powder with a known protein content of 24.38 wt%, and prepare a 4.0 mg / ml milk powder standard protein solution. Then a certain amount of bovine serum albumin (purchased from Roche Company) was weighed and prepared successively with the above milk powder standard protein ...

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Abstract

This invention relates to a kind of milk powder protein content detection kit, includes milk powder protein content standard color grade, sampler, transparence graduated vessels and biuret coloration liquid. The invention also open the manufacturing method of this kit, includes the step of make milk powder protein content standard color grade which as follows: use milk powder of given protein content and standard protein agent to prepare standard protein solution series contain milk powder; add biuret reagent into above series standard protein solution to obtain mixed liquor; shot mixed liquor;with image processing software to process photo, obtain color strip and output. This kit lends itself to carry. When detect milk powder protein content it not needs other supplementary instrument and equipment, detection cost low, testing time short. It has no special requirement to detection staff.

Description

technical field [0001] The invention relates to a kit for detecting protein content in milk powder. It specifically relates to a kit for measuring protein content in milk powder based on a biuret method using a colorimetric card, a manufacturing method thereof, and a detection method for protein content. Background technique [0002] Milk powder is an important food source for consumers, especially infants and young children. The level of its protein content is an important indicator of the quality of milk powder. The current determination method of protein content in milk powder adopts the Kjeldahl method specified in GB / T5413.1-1997 "Determination of protein in infant formula food and milk powder". The method is to heat and digest the milk powder sample with concentrated sulfuric acid and a catalyst to decompose the protein, in which carbon and hydrogen are oxidized to carbon dioxide and water to escape the reaction solution, while the organic nitrogen in the sample is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/25
Inventor 桑华春
Owner BEIJING ZHIYUNDA TECH CO LTD
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