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Method for preparing super-hole fibrin microsphere protein absorbing medium

A technology of cellulose microspheres and adsorption media, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of application limitations, low adsorption capacity, and poor pressure resistance, and achieve good environment and adsorption Large capacity and good pressure resistance

Inactive Publication Date: 2007-09-19
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of commercial cellulose adsorbents, such as poor pressure resistance and low adsorption capacity, limit its application in chromatographic separation processes.
Although this situation can be improved to a certain extent through the improvement of the preparation method, the average pore diameter of the spherical cellulose beads prepared by the spray method as Liu Minghua can reach 20-300nm (CN 1456593A), but its rapid separation of biomacromolecules Apps in still restricted

Method used

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  • Method for preparing super-hole fibrin microsphere protein absorbing medium
  • Method for preparing super-hole fibrin microsphere protein absorbing medium
  • Method for preparing super-hole fibrin microsphere protein absorbing medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] After 5 g of absorbent cotton was basified in 19% NaOH solution for 2 hours, the lye was squeezed out and placed in a water-bath shaker at 25°C for aging for 48 hours; the aged absorbent cotton was soaked in 2.5 mL of carbon disulfide (CS 2 ) after sulfonation for 10 hours, add 6% NaOH solution to dilute to 8% viscose solution, add 6g CaCO 3 , add this viscose solution to 600mL transformer oil containing compound emulsifier, and keep stirring at a stirring speed of 500rpm until it is dispersed into uniform droplets. The content of compound emulsifier in the oil phase is 0.4%, of which potassium oleate The mass ratio of Span60 and Span60 is 1:2.5, the dispersed system is placed in a super constant temperature water bath, the temperature is raised to 90°C, and the heat preservation is continued for 2.5 hours; after the material liquid is cooled, all the reaction mixture is poured out, let it stand for a while, and the upper layer of oil is centrifuged Pour off the phase l...

Embodiment 2

[0021] After 5 g of absorbent cotton was basified in 19% NaOH solution for 2 hours, the lye was squeezed out and placed in a water-bath shaker at 25°C for aging for 48 hours; the aged absorbent cotton was soaked in 2.5 mL of carbon disulfide (CS 2 ) after sulfonation for 8 hours, add 6% NaOH solution to dilute to 6% viscose solution, add 4gCaCO 3 , add this viscose solution to 600mL transformer oil containing compound emulsifier, and continue to stir and disperse at a stirring speed of 500rpm until the viscose is dispersed into uniform droplets. The mass ratio of potassium oleate and Span60 in the oil phase compound emulsifier The ratio is 1:2; the dispersed system is placed in a super constant temperature water bath, the temperature is raised to 90 ° C, and the temperature is continued for 2.5 hours; after the material liquid is cooled, the reaction mixture is poured out, and it is left to stand for a while, and the upper oil phase liquid is poured out by centrifugation. Wash...

Embodiment 3

[0024] Get 5g of the super-macroporous cellulose microsphere medium (calcium carbonate has been removed) prepared in Example 1, move it into a 250mL Erlenmeyer flask I, add 20mL 0.6mol / L DEAE-Cl to mix, and take another 250mL Erlenmeyer flask II Add 20mL of 3.5mol / L NaOH, seal it and place it in a constant temperature shaker at 60°C to preheat. After 10 minutes, add the NaOH in the Erlenmeyer flask II to the Erlenmeyer flask I and seal it. After reacting for 1 hour, the Erlenmeyer flask I was taken out and cooled with water. The modified macroporous medium was washed with deionized water in a sand core funnel until neutral.

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Abstract

The present invention discloses a preparation method for superporous cellulose microglobulin adsorption medium, which belongs to the preparation technology of cellulose gel medium of bioseparation engineering field. The method comprises the steps of: dispersing calcium carbonate particles to the cellulose viscose to form composite water phase; putting the viscose into the transformer oil and adding composite emulsifier potassium oleate and Span 60 and then executing agitating and emulsification; heating and curing to form particles; adding ethylene glycol diglycidyl ether and epichlorohydrin to execute double crosslinking reaction under alkaline condition; removing the calcium carbonate particles by using muriate acid and then using sodium borohydride to reducing so as to prepare superporous cellulose microglobulin adsorption medium. The medium not only maintains the advantages of the quondam cellulose gel medium but also increases a plurality of superporous, so that the resistance to pressure, the mass transfer rate and the dynamic adsorption capacity of the medium are all obviously improved, thereby, the novel superporous cellulose microglobulin adsorption medium has wide application prospect.

Description

technical field [0001] The invention relates to a preparation method of a super-macroporous cellulose microsphere protein adsorption medium, which belongs to the preparation technology of cellulose gel medium in the field of biological separation engineering. Background technique [0002] Due to its high separation precision and mild conditions, liquid chromatography technology is widely used in the preparation, separation and purification of biological substances such as proteins, and plays a pivotal role in the downstream processing of biological products. The development of new chromatographic media, its core technology, has also been It is one of the research hotspots in the field of chromatography. An ideal chromatography medium should not only have a high adsorption capacity, but also meet the requirement of maintaining high resolution at high flow rates. However, the pore diameter of most commercialized chromatographic media is only 10-200nm, which is close to the si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285B01J20/30C08J9/26C07K1/22
Inventor 孙彦王冬梅史清洪董晓燕白姝
Owner TIANJIN UNIV
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